PMID: 9188165Jan 1, 1997Paper

Further analysis of the role of spectrin repeat motifs in alpha-actinin dimer formation

European Biophysics Journal : EBJ
G FloodWalter Gratzer

Abstract

Protein constructs consisting of repeats 1-4, repeats 1-3 and repeats 2-4 of the rod domain of chicken alpha-actinin were expressed as fusion proteins in Escherichia coli. Based on the evidence of circular dichroism spectra and cooperative thermal unfolding profiles both truncated rod fragments were judged to have assumed the native structural fold. The thermal stabilities were in both cases significantly lower than that of the intact rod (repeats 1-4). Analyses by sedimentation equilibrium and velocity provided further evidence to show that fragment 1-4 is entirely dimeric in the concentration range of these experiments, resembling therefore the rod domain isolated by proteolytic digestion of native alpha-actinin. Fragment 2-4, and probably also 1-3, show concentration-dependent association, with dissociation constants, estimated by sedimentation equilibrium, in the 1-10 microM range. Thus, in confirmation of earlier work, all four repeats are required to generate a maximally stable anti-parallel dimer (Kd approximately 10 pM), suggesting the presence of binding sites in all of them to allow for aligned pairing.

Citations

Jun 29, 2012·Biochemistry·K S VishwanathaB A Eipper
Oct 28, 2015·The Journal of Cell Biology·Nivetha Kannan, Vivian W Tang
May 28, 2015·Science Signaling·Timothy TraversCarlos J Camacho
Feb 28, 2013·Biophysical Journal·Timothy TraversCarlos J Camacho
Jan 29, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Henni PiirainenVeli-Pekka Jaakola
Jun 29, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Daniel G MacArthur, Kathryn N North
Apr 21, 2005·The Journal of Biological Chemistry·Clara PratsThorkil Ploug
Oct 29, 1998·The Journal of Biological Chemistry·M Mazumdar, R A Cross
Feb 6, 2004·The Journal of Biological Chemistry·Richard LawDennis E Discher
Mar 17, 2010·Experimental Cell Research·Shyan Huey LowMaki Murata-Hori
Jul 24, 1998·Biochemical and Biophysical Research Communications·Y ChanL M Kunkel
Nov 4, 2004·Journal of Molecular Biology·Hideki KusunokiAlfonso Mondragón
Oct 13, 2005·Molecular and Biochemical Parasitology·Ana Virel, Lars Backman
Jan 11, 2011·Biochimica Et Biophysica Acta·Xiuli AnNarla Mohandas

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