PMID: 7539395Jun 1, 1995Paper

Further characterization and comparison of inducible nitric oxide synthase in mouse, rat, and human hepatocytes

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
A K NusslerT R Billiar

Abstract

Marked differences in induced nitric oxide (NO) synthesis occur between species. We have previously shown that both human and rat hepatocytes express an inducible NO synthase in response to cytokines and lipopolysaccharide. In this study, we compare the expression and regulation of cytokine-induced NO synthase in hepatocytes isolated from three species, human, rat, and mouse. On stimulation with tumor necrosis factor alpha (TNF alpha), interleukin-1 beta (IL-1 beta), interferon gamma (IFN gamma), and lipopolysaccharide (LPS), it was found that hepatocytes from all three species produce high levels of NO with levels of production exhibiting the following hierarchy: rat hepatocytes > mouse hepatocytes > human hepatocytes. Whereas rat and mouse hepatocytes express inducible NO synthase messenger RNA (mRNA) in response to TNF alpha, IL-1 beta, or IFN gamma as a single stimulus, human hepatocytes respond to LPS alone. Inhibition of NO generation through transforming growth factor (TGF-beta 1) was seen in mouse (77% +/- 5.9) and rat hepatocytes (17% +/- 2.6) whereas only about 10% was seen in human hepatocytes. Epidermal growth factor (EGF) was shown to inhibit NO synthesis in human and mouse hepatocytes but not rat. A marked NO-depe...Continue Reading

References

Feb 14, 1992·Biochemical and Biophysical Research Communications·F Q CunhaF Y Liew
Jul 1, 1992·The Journal of Experimental Medicine·A K NusslerR L Simmons
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinS H Snyder
Jul 31, 1992·Biochemical and Biophysical Research Communications·T B McCallS Moncada
Feb 11, 1991·Current Opinion in Immunology·C F Nathan, J B Hibbs
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D J StuehrC F Nathan
Oct 1, 1991·The Journal of Experimental Medicine·N S KwonC F Nathan
May 16, 1990·Biochemical and Biophysical Research Communications·T R BilliarS A Murray
Nov 1, 1989·The Journal of Experimental Medicine·R D CurranR L Simmons
Dec 1, 1989·Archives of Surgery·T R BilliarR L Simmons
Jun 15, 1989·Biochemical and Biophysical Research Communications·D J StuehrC F Nathan
Apr 1, 1989·The Journal of Experimental Medicine·T R BilliarR L Simmons
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J Stuehr, M A Marletta
Jan 1, 1974·Analytical Biochemistry·J L Corbin, M Reporter
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
Mar 31, 1993·Biochemical and Biophysical Research Communications·E R WoodE G Lapetina
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
Aug 1, 1993·The Journal of Experimental Medicine·Y VodovotzC Nathan
Aug 1, 1993·The Journal of Experimental Medicine·N McCartney-FrancisS M Wahl
Aug 31, 1993·Biochemical and Biophysical Research Communications·R S Gilbert, H R Herschman

❮ Previous
Next ❯

Citations

Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R A SchroederP C Kuo
Aug 8, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Z WangB I Carr
Aug 10, 2006·Pediatric Surgery International·Abdurrahman KaramanErsin Fadillioglu
Feb 4, 2010·Pediatric Surgery International·Abdurrahman KaramanMehmet Demircan
Nov 1, 1995·Research in Immunology·A K NusslerT R Billiar
Oct 3, 2001·Journal of Hepatology·P OlingaG M Groothuis
Jun 24, 1998·International Journal of Immunopharmacology·H FarghaliK Masek
Jul 11, 1998·Critical Care Medicine·C M Pastor, P M Suter
Jan 15, 2011·Journal of Leukocyte Biology·David A WinkCarol A Colton
Oct 12, 2000·Biochemical and Biophysical Research Communications·D A GellerK Hatakeyama
Sep 9, 1999·In Vitro Cellular & Developmental Biology. Animal·A K NusslerS C Strom
Mar 19, 2011·Current Opinion in Cardiology·Apurva V Shirodkar, Philip A Marsden
Dec 22, 1999·The Journal of Physiology·S R BartlettR Poston
Feb 1, 1997·Biochemical and Molecular Medicine·J B Domachowske
Aug 2, 2001·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·K PaillaF Blonde-Cynober
Jun 1, 1997·Nitric Oxide : Biology and Chemistry·F BlachierO Soubrane
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M J AmaroV Carreño
Apr 26, 2008·Circulation Research·Charles C Matouk, Philip A Marsden
Mar 27, 2001·European Journal of Clinical Investigation·N DikopoulosH Weidenbach

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.