Further characterization of benzo[a]pyrene diol-epoxide (BPDE)-induced comet assay effects

Mutagenesis
Julia BausingerGünter Speit

Abstract

The present study aims to further characterize benzo[a]pyrene diol-epoxide (BPDE)-induced comet assay effects. Therefore, we measured DNA effects by the comet assay and adduct levels by high-performance liquid chromatography (HPLC) in human lymphocytes and A549 cells exposed to (±)-anti-benzo[a]pyrene-7,8-diol 9,10-epoxide [(±)-anti-BPDE] or (+)-anti-benzo[a]pyrene-7,8-diol 9,10-epoxide [(+)-anti-BPDE]. Both, the racemic form and (+)-anti-BPDE, which is the most relevant metabolite with regard to mutagenicity and carcinogenicity, induced DNA migration in cultured lymphocytes in the same range of concentrations to a similar extent in the alkaline comet assay after exposure for 2h. Nevertheless, (+)-anti-BPDE induced significantly enhanced DNA migration after 16 and 18h post-cultivation which was not seen in response to (±)-anti-BPDE. Combination of the comet assay with the Fpg (formamidopyrimidine-DNA glycosylase) protein did not enhance BPDE-induced effects and thus indicated the absence of Fpg-sensitive sites (oxidized purines, N7-guanine adducts, AP-sites). The aphidicolin (APC)-modified comet assay suggested significant excision repair activity of cultured lymphocytes during the first 18h of culture after a 2 h-exposure to B...Continue Reading

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Citations

Jun 7, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Günter SpeitJulia Bausinger
Jul 9, 2016·Environmental Toxicology and Pharmacology·Soudabeh ImanikiaVolker M Arlt
Aug 2, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Heloísa Lizotti CiliãoIlce Mara de Syllos Cólus
Jul 6, 2016·Archives of Toxicology·M GleiW Schlörmann
Oct 6, 2021·Archives of Toxicology·Ezgi Eyluel BankogluHelga Stopper

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