PMID: 698205Sep 11, 1978Paper

Further characterization of calcium-accumulating vesicles from human blood platelets

Biochimica Et Biophysica Acta
R Käser-GlanzmannE F Lüscher

Abstract

Human blood platelets are capable of removing Ca2+ from the cytoplasm by means of an active, ATP-dependent and cyclic AMP-stimulated transport system. Calcium-accumulating vesicles are obtained by sonicating platelets. On density gradient centrifugation, this activity is found in the heavier of two membrane fractions. Concentrated in this fraction are also the Ca2+-stimulated Mg2+-ATPase and glucose-6-phosphatase, believed to be a marker for internal membrane systems. When the isolated vesicles are loaded with Ca2+, a third band separates from the two vesicular fractions in the density gradient. This band C contains virtually all the Ca2+-accumulating activity. Evidence that this activity is due to an active uptake and not to surface binding or adsorption is presented. Whereas electron microscopy does not reveal striking differences between active and inactive fractions, differences in protein composition are revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Furthermore, this band contains an enzyme system which converts arachidonic acid to malondialdehyde and therefore this fraction must be the site of prostaglandin synthesis. Membranes prepared by loading platelets with glycerol, followed by osmotic lysi...Continue Reading

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Citations

Jan 1, 1982·Pharmacology & Therapeutics·N G Ardlie
Feb 16, 2000·Thrombosis Research·T M WuT C Chou
Oct 13, 2012·The Journal of Biological Chemistry·Adam J WieschhausAthar H Chishti
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Sep 10, 1985·Biochimica Et Biophysica Acta·B Steiner, E F Lüscher
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Aug 30, 1984·Biochemical and Biophysical Research Communications·J EnoufS Levy-Toledano

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