Further Characterization of Molecular Markers in Canine Dirofilaria immitis Infection

The Journal of Parasitology
Ashley BramanCharles Mackenzie

Abstract

Dirofilaria immitis is a common filarial parasite found in dogs and cats in the Americas, with the pathophysiological consequences of the infection differing somewhat between these 2 host species. Recent research efforts have been focused on determining if the microRNAs (miRNAs) released from adult Dirofilariae have a role as markers for distinguishing the intensity of adult worm infection, as well as determining the presence of new infections. This study expands previous work on 2 nematode miRNAs, miR34 and miR-71, by addressing their ability to discriminate between low and high D. immitis adult worm intensities in dogs. Serum samples were collected from 13 dogs, 8 of which carried known numbers of adult D. immitis at autopsy in their hearts and pulmonary vessels. Three groups of canine sera were created based on D. immitis burden: "control" (0 worms; 5 animals), "low intensity" (10-18 worms; mean ± SD = 12.3 ± 4.4; 4 animals), and "high intensity" (41-72 worms; mean 62.5 ± 15.1; 4 animals) groups. A qPCR analysis was performed on each sample to measure plasma levels of miR-34 and miR-71; however, no significant differences were observed between these groups in terms of levels of miRNAs, so the low- and high-intensity samples ...Continue Reading

References

May 20, 2008·Advances in Parasitology·John W McCallLuigi Venco
Nov 26, 2009·The Veterinary Clinics of North America. Small Animal Practice·Dwight D Bowman, Clarke E Atkins
Dec 7, 2010·Current Biology : CB·Alexandre de LencastreFrank J Slack
Jun 15, 2011·Veterinary Immunology and Immunopathology·G GioiaS Comazzi
Jun 19, 2013·Journal of Internal Medicine·M HaneklausS L Masters
Jul 18, 2014·PLoS Neglected Tropical Diseases·Lucienne TrittenTimothy Geary
Jul 21, 2016·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·Gloria BertoliIsabella Castiglioni

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Citations

Mar 28, 2021·Molecular Immunology·Marcela A CucherLaura Kamenetzky

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Methods Mentioned

BETA
PCR

Software Mentioned

SAS

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