PMID: 44945Dec 1, 1979

Further characterization of "promptly" and "delayed" human serum-sensitive strains of Serratia marcescens

Zentralblatt Für Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie Und Parasitologie
W H Traub, P I Fukushima

Abstract

The kinetics of the bactericidal activity of 80 vol% of fresh human serum against representative 'delayed serum-sensitive' (DSS) and 'promptly serum-sensitive' (PSS) strains of Serratia marcescens were further examined with regard to various chemical and absorption procedures known to affect various components of the alternative and classical pathways of human complement activation. Inulin treatment of fresh human serum failed to diminish serum bactericidal activity against DSS and PSS assay strains. Fresh human serum that had been depleted of properdin (factor P) through absorption with zymosan, was as active as control serum against DSS strains of S. marcescens; however, PSS strains were killed in a 'delayed' fashion. Human serum that had been heat-inactivated at 50 degrees C for minutes (depletion of factor B), no longer killed DSS strains, whereas PSS strains of S. marcescens and the PSS control strain Escherichia coli C were killed in a slightly delayed fashion. Hydrazine-hydrate treatment (inactivation of C3 of the complement system) and exposure of fresh human serum to dithiothreitol completely abolished serum bactericidal activity. Bentonite-absorbed fresh human serum no longer killed DSS strains of S. marcescens; some ...Continue Reading

Related Concepts

Alkalescens-Dispar Group
Serratia marcescens
LYZ
Process of Absorption
Sputolysin
Hydrazines
Insulin Resistance
Inulin
Zymosan
Blood Bactericidal Activity

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