PMID: 9532266Apr 9, 1998Paper

Further characterization of Staphylococcus epidermidis transposon mutants deficient in primary attachment or intercellular adhesion

Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology
C Heilmann, F Götz

Abstract

Biofilm formation is suggested to be the result of primary attachment of Staphylococcus epidermidis cells to a polymer surface followed by accumulation in multilayered cell clusters. Here we describe the further characterization of transposon (Tn917) mutants of Staphylococcus epidermidis O-47 having been biofilm-negative in a polystyrene microtiter plate adhesion assay. Among 5000 Tn917 insertion strains, we isolated four biofilm-negative mutants, each carrying one copy of Tn917. The mutants could be divided into two phenotypic classes: class A (mut1 and mut1a) and class B (mut2 and mut2a). Mutants of phenotype class A lacked four cell surface proteins and were affected in the primary attachment to polystyrene, but remained able to form multilayered cell clusters and to produce PIA. Mutants of phenotype class B were able to attach to polystyrene, but did not form multilayered cell clusters nor produce PIA. The cell surface protein pattern relative to the wild type was unchanged in class B mutants. On Congo red agar, the wild type and class A mutants formed black colonies (positive reaction on Congo red agar) while class B mutant colonies were red (negative reaction). The initial binding of cells to polystyrene and the ability t...Continue Reading

References

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Citations

Apr 7, 2006·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R BradfordM Deighton
Jun 25, 2010·Journal of Industrial Microbiology & Biotechnology·J S PalmerJ D Brooks
Nov 7, 2000·FEMS Immunology and Medical Microbiology·S LangT S Elliott
Nov 1, 2002·The Lancet Infectious Diseases·Christof von EiffChristine Heilmann
May 5, 2005·Applied and Environmental Microbiology·Alexandra ReschFriedrich Götz
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Feb 13, 2015·Frontiers in Cellular and Infection Microbiology·Hannah McCarthyJames P O'Gara
Nov 19, 2002·FEMS Microbiology Letters·Kevin M ConlonJames P O'Gara
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May 10, 2017·The Journal of Infectious Diseases·Sheetal TrivediFranklin D Lowy
Apr 16, 2002·Molecular Microbiology·Friedrich Götz
Aug 26, 2006·Periodontology 2000·Mary E Davey, John W Costerton
May 18, 2006·The International Journal of Artificial Organs·N HarraghyM Herrmann
May 16, 2002·Periodontology 2000·Sigmund S Socransky, Anne D Haffajee
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·W W Navarre, O Schneewind

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