Further characterization of the mouse sperm surface zona-binding site with galactosyltransferase activity

Molecular Reproduction and Development
D A BenauB T Storey

Abstract

One of the mouse sperm surface binding sites for zona pellucida ligands exhibits galactosyltransferase (GT) enzyme activity. The present study was undertaken to ascertain whether the GT site behaves as a noncatalytic binding site in its physiological capacity, with no glycosylation of zona ligands, or whether glycosylation of zona ligands is an integral part of sperm-zona binding. The effects of Mn2+, the obligatory cation for GT catalysis, on enzyme activity and sperm-zona binding were examined. With uridine-5'-diphosphogalactose (UDPgal) as galactose donor, and N-acetylglucosamine (GlcNAc) as galactose acceptor, increasing concentrations of Mn2+ in the range of 0.1-10 mM increased GT enzyme activity, with half-maximal activation at 0.65 mM Mn2+ (Vmax = 20 pmol/hr/10(6) cells). In the presence of 0-2 mM Mn2+, sperm-zona binding was inhibited in a concentration-dependent manner; 50% inhibition occurred at 1.25 mM Mn2+. At this concentration, GT enzyme activity was at 65% Vmax. To determine the specificity of the GT site for glycoprotein terminal carbohydrate residues, spermatozoa were incubated with, asialo-ovine submaxillary mucin (N-acetylgalactosamine residues), asialo-, -alpha 1-acid glycoprotein (beta 1-4 galactose residue...Continue Reading

References

Aug 1, 1978·Developmental Biology·P M SalingD P Wolf
Feb 10, 1977·Nature·R DurrS Roth
Oct 1, 1977·The Journal of Cell Biology·R B Marchase
Jun 1, 1975·Developmental Biology·R M PrattM C Johnston
Dec 1, 1987·Developmental Biology·J D McLaughlin, B D Shur
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J D Bleil, P M Wassarman
Oct 1, 1985·The Journal of Cell Biology·L C LopezB D Shur
Nov 1, 1986·Molecular and Cellular Biochemistry·E M BaynaB D Shur
Jan 1, 1986·Advances in Experimental Medicine and Biology·B D Shur
Dec 1, 1973·The Quarterly Review of Biology·S Roth
Nov 3, 1971·Nature: New Biology·G A JamiesonA J Barber
Nov 1, 1971·The Journal of Cell Biology·S RothS Roseman
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S Roth, D White
Nov 1, 1984·Developmental Biology·H M FlormanP M Wassarman
Nov 1, 1982·The Journal of Cell Biology·B D Shur, N G Hall
Apr 1, 1980·The Journal of Experimental Zoology·L J HeffnerB T Storey
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P M Saling
Jan 1, 1980·International Review of Cytology·M PierceS Roth

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Citations

Dec 1, 1996·Molecular Reproduction and Development·C D Thaler, R A Cardullo
Jan 5, 2002·Molecular Reproduction and Development·Lucile MontfortRobert Sullivan
Oct 8, 2009·The Journal of Reproduction and Development·Yosuke SakaguchiYasunori Monji
Jul 24, 2008·The International Journal of Developmental Biology·Barry D Shur
Dec 22, 1998·Biology of Reproduction·C R Loeser, D R Tulsiani
Nov 1, 1995·Andrologia·E Töpfer-PetersenF Sinowatz
Oct 24, 1998·Biochemical and Biophysical Research Communications·B D Shur
Jun 23, 2021·Nature Chemistry·John F SeelerThomas V O'Halloran

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