Further characterization of the murine collagenase (type IVB) gene promoter and analysis of mRNA expression in murine tissues

Gene
J RoachS V Reddy

Abstract

The collagenase B type IV (Col4B) gene is highly expressed in the osteoclast, the primary bone-resorbing cell. However, factors that regulate expression of the Col4B gene are not well characterized. A murine P1 genomic clone containing a 94 kb sequence insert which contains the Col4B gene was isolated. A 4 kb EcoR1 DNA fragment containing the 5' flanking sequence of the gene was further subcloned and restriction mapped. Putative transcription factors such as SRY, Lyf-1, and GATA1 and 2, binding motifs were identified by sequence analysis in this promoter region. Enhancer and suppressor regions were mapped by transient expression of Col4B gene promoter deletion mutant-luciferase reporter gene constructs in HepG2 cells. Col4B mRNA expression in different murine tissues was analyzed by reverse transcription-polymerase chain reaction and demonstrated high levels of expression in bone, clavaria, spleen and thymus. This promoter provides a valuable tool for targeting gene expression to the osteoclast.

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Citations

Sep 23, 2003·Journal of Cellular Physiology·Robert M GreeneM Michele Pisano
Apr 1, 2008·Chemical Biology & Drug Design·Kunal Roy, Partha Pratim Roy
Sep 30, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Landry K KamdemZeruesenay Desta
Jun 1, 2014·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Brahim AchourAmin Rostami-Hodjegan
Oct 5, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Corie A EllisonJames R Olson
Dec 15, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Katarina IlicMei-Ling Chen
Jul 3, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Jian YuDoug Burdette
Aug 19, 2011·The Journal of Pharmacology and Experimental Therapeutics·Shotaro UeharaYasuhiro Uno
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer L OwenDiana M Lopez
Sep 14, 1999·The American Journal of Physiology·R C PereiraE Canalis

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