PMID: 2092074Oct 1, 1990Paper

Further characterization of the process of in vitro uptake of radiolabeled copper by the rat brain

Journal of Inorganic Biochemistry
A BarneaM D Edwards

Abstract

We have previously demonstrated that hypothalmic slices obtained from adult male rats accumulate 67Cu by two ligand-dependent, saturable processes: a high and low affinity process. To further establish the generality of these uptake processes, we defined the ligand requirements and the saturation kinetics of 67Cu uptake by tissue slices obtained from the newborn hypothalamus (HT); adult male hypothalamus, hippocampus, cortex, median eminence, and caudate nucleus; hypothalamus and hippocampus of castrated (14 days) males and of pregnant (19 days) and ovariectomized (14 days) females. It was found that ionic 67Cu2+ was poorly taken up by newborn HT and adult caudate, complexation with His enhanced 67Cu uptake 3-4-fold, and complexation with albumin inhibited 67Cu uptake. These ligand requirements are identical to those we have previously shown for the adult HT. When 67Cu uptake was evaluated under conditions optimal for the high or the low affinity process, for each process the dose response curves generated from these various tissues were very similar. In addition, we assessed the uptake of both components of the CuHis2 complex by incubating tissues with 67Cu3 H-His2 and found that the tissue ratio of 67Cu:3H was a sigmoidal fun...Continue Reading

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Citations

Jan 31, 2012·Journal of Toxicology·Sanah SadiqDietrich Büsselberg
Oct 29, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ya Hui HungRobert Alan Cherny
Sep 7, 1999·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·J H Menkes
Jan 24, 2003·Expert Opinion on Therapeutic Targets·Hiroko KodamaMakiko Mizunuma
Oct 1, 1993·European Journal of Pediatrics·J KreuderD Dralle
Jul 5, 2018·Journal of Neurochemistry·Felix NeumaierToni Schneider
Mar 19, 2014·Journal of Labelled Compounds & Radiopharmaceuticals·Rebekka Hueting
Feb 27, 1997·Biochimica Et Biophysica Acta·M DiDonato, B Sarkar
May 1, 1992·Medical Hypotheses·H A Hartmann, M A Evenson

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