PMID: 6736855Jul 1, 1984Paper

Further characterization of the second oestrogen-binding species of the rat granulosa cell

The Journal of Endocrinology
G B KudoloL Myatt

Abstract

Rat granulosa cell cytosol contains a second oestrogen-binding species (SOB) distinguished from the classical oestrogen receptor by its lower dissociation constant (approx. 45 nmol/l) and the ability to bind oestrogens, antioestrogens, androgens and progesterone but not diethylstilboestrol. The SOB and the oestrogen receptor can be further distinguished by their differential adsorption to spheroidal hydroxylapatite and Concanavalin A-Sepharose. Addition of chaotropic salts or molybdate to granulosa cell cytosol did not alter the concentration of SOB or oestrogen receptor measured, indicating that there are no 'masked' binding sites in the two species caused by aggregation phenomena. The association rate of oestradiol with SOB at 4 degrees C (1.72 +/- 0.27(S.E.M.) X 10(8) mol/h) and 25 degrees C (4.50 +/- 0.36 X 10(8) mol/h) was faster than with the oestrogen receptor (7.20 +/- 0.15 X 10(7) mol/h and 1.23 +/- 0.15 X 10(8) mol/h respectively). The biphasic dissociation kinetics of [3H]oestradiol from the oestrogen receptor at 25 degrees C (rate constants k-1 = 0.30 +/- 0.07/min and k-2 = 3.73 +/- 0.57 X 10(-3)/min) were similar to those reported in other target tissues but the dissociation of [3H]oestradiol from SOB appeared to b...Continue Reading

Citations

Jun 16, 2001·Endocrine Reviews·S F PalterE Y Adashi
Oct 1, 1986·Archives of Biochemistry and Biophysics·B J Rogerson, P K Eagon

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