Further definition of the sequence and position requirements of the arginine control element that mediates repression and induction by arginine in Saccharomyces cerevisiae

Yeast
M CrabeelA Matisova

Abstract

Repression or induction of the genes involved in arginine biosynthesis or catabolism, respectively, both require participation of the ArgRp/Mcm1p regulatory complex. Our previous work showed that those opposite effects were mediated by a similar arginine-responsive element of 23 nucleotides (that we now call ARC, for ARginine Control) situated close to the start of transcription in the repressed promoters and far upstream of the TATA-element in the induced promoters. To define more precisely the sequence and position requirements of the ARC element, we have now characterized by mutagenesis the promoter elements of the arginine-repressible ARG1 and ARG8 genes. We also identify a functional ARC in the CPA1 promoter, thereby confirming, in agreement with our previous mRNA pulse-labelling data, the participation of a transcriptional component in the arginine regulation of that gene otherwise submitted to a translational regulation. From the 12 ARC elements now characterized, we have derived a consensus sequence and show that such a synthetic element is able to mediate ArgRp/Mcm1p-dependent arginine regulation. An important new finding illustrated by ARG1 and CPA1, is that contrary to what all the previous data suggested, repression...Continue Reading

References

Mar 1, 1979·European Journal of Biochemistry·F Ramos, J M Wiame
Apr 1, 1991·Molecular and Cellular Biology·E Dubois, F Messenguy
Jan 1, 1986·CRC Critical Reviews in Biochemistry·A G Hinnebusch
Jun 1, 1993·Current Opinion in Cell Biology·K Struhl

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Citations

Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ling-ju Lin, Michael C Schultz
Aug 15, 2009·The Journal of Biological Chemistry·Lorena Peiró-Chova, Francisco Estruch
May 21, 2005·Nucleic Acids Research·Zhihong Zhang, Fred S Dietrich
Nov 13, 2012·Eukaryotic Cell·Claudia Jiménez-LópezMichael C Lorenz
Jan 19, 2012·Molecular and Cellular Biology·Elia M Crisucci, Karen M Arndt
May 25, 2002·Molecular and Cellular Biology·Suzanne D TurnerChristopher J Brandl
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sungpil YoonAlan G Hinnebusch
Feb 24, 2009·Biochemical and Biophysical Research Communications·Sungpil Yoon, Alan G Hinnebusch
Jun 1, 1996·Microbiological Reviews·P S Lovett, E J Rogers

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