Abstract
To date, there has been no well-established local lymph node assay (LLNA) that includes an elicitation phase. Therefore, we developed a modified local lymph node assay with an elicitation phase (LLNA:DAE) to discriminate true skin sensitizers from chemicals that gave borderline positive results and previously reported this assay. To develop the LLNA:DAE method as a useful stand-alone testing method, we investigated the complete procedure for the LLNA:DAE method using hexyl cinnamic aldehyde (HCA), isoeugenol, and 2,4-dinitrochlorobenzene (DNCB) as test compounds. We defined the LLNA:DAE procedure as follows: in the dose-finding test, four concentrations of chemical applied to dorsum of the right ear on days 1, 2, and 3 and dorsum of both ears on day 10. Ear thickness and skin irritation score were measured on days 1, 3, 5, 10, and 12. Local lymph nodes were excised and weighed on day 12. The test dose for the primary LLNA:DAE study was selected as the dose that gave the highest left ear lymph node weight in the dose-finding study, or the lowest dose that produced a left ear lymph node of over 4 mg. This procedure was validated using nine different chemicals. Furthermore, qualitative relationship was observed between the degree ...Continue Reading
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Citations
Mar 23, 2017·The Journal of Toxicological Sciences·Hongwei ZhangXiaoyan Yang
Aug 7, 2018·The Journal of Toxicological Sciences·Kunihiko YamashitaHiroshi Itagaki
Nov 5, 2019·Journal of Applied Toxicology : JAT·Sun-A ChoJae-Hak Park
Nov 13, 2015·The Journal of Toxicological Sciences·Kunihiko YamashitaHiroshi Itagaki
Aug 21, 2019·Regulatory Toxicology and Pharmacology : RTP·Renato Ivan de ÁvilaMarize Campos Valadares