Further evaluation of the NWF filter for the purification of Plasmodium vivax-infected erythrocytes

Malaria Journal
Jiangyan LiLiwang Cui

Abstract

Isolation of Plasmodium-infected red blood cells (iRBCs) from clinical blood samples is often required for experiments, such as ex vivo drug assays, in vitro invasion assays and genome sequencing. Current methods for removing white blood cells (WBCs) from malaria-infected blood are time-consuming or costly. A prototype non-woven fabric (NWF) filter was developed for the purification of iRBCs, which showed great efficiency for removing WBCs in a pilot study. Previous work was performed with prototype filters optimized for processing 5-10 mL of blood. With the commercialization of the filters, this study aims to evaluate the efficiency and suitability of the commercial NWF filter for the purification of Plasmodium vivax-infected RBCs in smaller volumes of blood and to compare its performance with that of Plasmodipur®filters. Forty-three clinical P. vivax blood samples taken from symptomatic patients attending malaria clinics at the China-Myanmar border were processed using the NWF filters in a nearby field laboratory. The numbers of WBCs and iRBCs and morphology of P. vivax parasites in the blood samples before and after NWF filtration were compared. The viability of P. vivax parasites after filtration from 27 blood samples was e...Continue Reading

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Citations

Aug 28, 2020·Frontiers in Microbiology·Awtum M BrashearLiwang Cui
Aug 7, 2017·Trends in Parasitology·Richard Thomson-LuqueErica M Pasini
Nov 19, 2021·Frontiers in Cellular and Infection Microbiology·Weilin ZengZhaoqing Yang

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Methods Mentioned

BETA
density gradient centrifugation
column filtration

Software Mentioned

Plasmodipur
SPSS

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