Further evidence that inhibitor-2 acts like a chaperone to fold PP1 into its native conformation

FEBS Letters
C MacKintoshP Cohen

Abstract

The gamma1-isoform of protein phosphatase-1 expressed in Escherichia coli (PP1gamma) and the native PP1 catalytic subunit (PP1C) isolated from skeletal muscle dephosphorylated Ser-14 of glycogen phosphorylase at comparable rates. In contrast, PP1gamma dephosphorylated several tyrosine-phosphorylated proteins at similar rates to authentic protein tyrosine phosphatases (PTPases), but native PP1C was almost inactive towards these substrates. The phosphorylase phosphatase (PhP) and PTPase activities of PP1gamma were inhibited by vanadate with IC50 values (30-100 microM) comparable to authentic PTPases, whereas the PhP activity of native PP1C was insensitive to vanadate. PP1gamma lost its PTPase activity, and its PhP activity became insensitive to vanadate, after interaction with inhibitor-2, followed by the reversible phosphorylation of inhibitor-2 at Thr-72. These findings support and extend the hypothesis that inhibitor-2 functions like a chaperone to fold PP1 into its native conformation, and suggest that the correct folding of PP1 may be critical to prevent the uncontrolled dephosphorylation of cellular phosphotyrosine residues.

References

Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Jan 1, 1988·Methods in Enzymology·P CohenN K Tonks
Jan 15, 1993·European Journal of Biochemistry·M RuzzeneL A Pinna
Aug 1, 1994·Trends in Cell Biology·R E Mayer-Jaekel, B A Hemmings

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Citations

Apr 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hugo CeulemansMathieu Bollen
Nov 19, 2003·Biochemical and Biophysical Research Communications·Jun O Liu
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·T WatanabeA C Nairn
Nov 24, 2004·BMC Evolutionary Biology·Alexandra V Andreeva, Mikhail A Kutuzov
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Persoulla Nicolaou, Evangelia G Kranias
Jan 15, 2014·Nature Structural & Molecular Biology·Amelie SchreieckLori A Passmore
Jan 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rick B VegaEric N Olson
May 31, 2013·Biology of the Cell·Nicole DeVaulAnn O Sperry
Apr 24, 2012·The FEBS Journal·David L Brautigan
Jan 31, 2012·The FEBS Journal·Wolfgang PetiRebecca Page
Jun 7, 2000·The Journal of Biological Chemistry·H C Wang, K A Fecteau
Sep 11, 2002·The Journal of Biological Chemistry·Masumi EtoDavid L Brautigan
Jan 13, 2004·Physiological Reviews·Hugo Ceulemans, Mathieu Bollen
Oct 31, 2020·Biochemical Society Transactions·Sarah Lemaire, Mathieu Bollen
Feb 7, 2006·International Journal of Biological Macromolecules·Thomas Doohun Kim
Dec 25, 2019·Journal of Medicinal Chemistry·Sayumi YamazoeSteven T Staben
Dec 26, 2001·Chemical Reviews·P J Kennelly

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