Further exploration of the conformational space of α-synuclein fibrils: solid-state NMR assignment of a high-pH polymorph

Biomolecular NMR Assignments
Joeri VerasdonckBeat H Meier

Abstract

Polymorphism is a common and important phenomenon for protein fibrils which has been linked to the appearance of strains in prion and other neurodegenerative diseases. Parkinson disease is a frequently occurring neurodegenerative pathology, tightly associated with the formation of Lewy bodies. These deposits mainly consist of α-synuclein in fibrillar, β-sheet-rich form. α-synuclein is known to form numerous different polymorphs, which show distinct structural features. Here, we describe the chemical shift assignments, and derive the secondary structure, of a polymorph that was fibrillized at higher-than-physiological pH conditions. The fibrillar core contains residues 40-95, with both the C- and N-terminus not showing any ordered, rigid parts. The chemical shifts are similar to those recorded previously for an assigned polymorph that was fibrillized at neutral pH.

References

Mar 15, 2002·The Journal of Biological Chemistry·Hirotomo MiakeMasato Hasegawa
Jun 20, 2003·The Journal of Biological Chemistry·Ani Der-SarkissianRalf Langen
Oct 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Henrike HeiseMarc Baldus
Sep 26, 2009·Journal of Biomolecular NMR·Anja BöckmannAnne Lesage
Mar 24, 2010·Nature Reviews. Molecular Cell Biology·Patrik BrundinRon Kopito
Jun 24, 2010·Chembiochem : a European Journal of Chemical Biology·Anne SchuetzBeat H Meier
Jan 20, 2011·The Journal of Clinical Investigation·Christian HansenPatrik Brundin
Feb 24, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Michael Eller, David R Williams
Jul 2, 2011·Journal of Molecular Biology·Gemma ComellasChad M Rienstra
Jul 12, 2011·Biomolecular NMR Assignments·Julia GathAnja Böckmann
Aug 2, 2011·Journal of Biomolecular NMR·Birgit HabensteinAnja Böckmann
Sep 29, 2011·Journal of Biomolecular NMR·Tim J StevensErnest D Laue
Feb 23, 2012·Journal of the American Chemical Society·Gemma ComellasChad M Rienstra
Apr 21, 2012·Journal of Molecular Biology·Guohua LvAdam Lange
Oct 11, 2013·Nature Communications·Luc BoussetRonald Melki
Oct 12, 2013·Biomolecular NMR Assignments·Julia GathAnja Böckmann

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Citations

Sep 22, 2016·Critical Reviews in Biochemistry and Molecular Biology·Julia E GersonRakez Kayed
Jan 12, 2017·The Journal of Physical Chemistry. B·Carolin SeuringRoland Riek
Jan 24, 2018·Journal of Internal Medicine·M FändrichP Hammarström
May 21, 2017·Acta Neuropathologica·William P FlavinEdward M Campbell
Dec 1, 2020·Biophysical Chemistry·Magdalena I IvanovaAyyalusamy Ramamoorthy

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