PMID: 8596713Feb 1, 1996Paper

Further investigation of ionic diffusive properties and of NH4+ pathways in Xenopus laevis oocyte cell membrane

Pflügers Archiv : European journal of physiology
Marc CougnonGabrielle Planelles

Abstract

To study the ionic diffusive properties and the NH4+ pathways in the Xenopus laevis oocyte cell membrane, we recorded the effects of various inhibitors on membrane potential (Vm) and membrane resistance (Rm); intracellular acidification was taken as an index of NH4+ influx from the bath to the cytoplasm. The following results were obtained: in the control state, barium and quinine (Q) ions depolarized Vm and raised Rm, consistent with inhibition of K+ conductance(s). Diphenylamine-2-carboxylic acid (DPC), 3',5'-dichlorodiphenylamine-2-carboxylic acid (DCDPC) and gadolinium ions hyperpolarized Vm and increased Rm, suggesting the inhibition of nonselective cationic conductance(s). In the presence of 20 mmol/l NH4Cl, Vm depolarized, Rm fell, and intracellular pH (pHi) decreased, consistent with an NH4+ influx. In the presence of DPC, the same manoeuvre induced a biphasic Vm change (i.e. a spike depolarization followed by a membrane hyperpolarization) and a fall of Rm; in most oocytes, intracellular acidification persisted and was reversible upon adding ouabain (Oua). These results indicate that a DPC-sensitive conductance is not the unique NH4+ pathway and that Na, K-ATPase may also mediate NH4+ influx. However, Oua did not preven...Continue Reading

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Citations

Aug 19, 2010·The Journal of Membrane Biology·Matthieu MarinJean-Pierre Vilain
Jul 1, 2005·Pflügers Archiv : European journal of physiology·Lars M HolmThomas Zeuthen
Nov 29, 2007·Pflügers Archiv : European journal of physiology·Rikke Søgaard, Thomas Zeuthen
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