PMID: 9429301Jun 1, 1997Paper

Further similarities between astrocytes and perisinusoidal stellate cells of liver (Ito cells): colocalization of desmin and glial fibrillary acidic protein in astroglial primary cultures

Biology of the Cell
G H Buniatian

Abstract

The colocalization of desmin and glial fibrillary acidic protein (GFAP) in astrocytes was inferred from previous studies demonstrating a unique antigenic composition comprising GFAP, desmin and vimentin in perisinusoidal stellate cells (PSC) of liver which share several features with astrocytes. In the present study the colocalization of GFAP and desmin was investigated by double-immunolabeling experiments in 12-day-old rat astroglial primary cultures with antiserum against GFAP and two commercial monoclonal antibodies against desmin, antibodies of clone DEU-10 and clone DEB-5. These antibodies selectively decorated the perisinusoidal stellate cells (PSC) of liver for which desmin is known to be a marker. The results obtained with astroglial cells demonstrate that both GFAP and desmin are coexpressed in morphologically different types, process-bearing and process-lacking astrocytes. The expression of desmin was apparently more pronounced in process-lacking astrocytes and was considerably lower in process-bearing ones. In process-lacking astrocytes, in contrast to filamentous cytoplasmic staining for GFAP, the immunoreactivity for desmin was non-filamentous and was irregularly spread in the perinuclear cytoplasm of the cells, wh...Continue Reading

Citations

Jan 3, 2013·Acta Ophthalmologica·Alice L Yu, Ulrich Welge-Lussen
May 2, 2008·The International Journal of Biochemistry & Cell Biology·Arancha Botella-LopezJavier Sáez-Valero

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