PMID: 7539644Feb 1, 1995Paper

Further studies of the binding specificity of the leukocyte adhesion molecule, L-selectin, towards sulphated oligosaccharides--suggestion of a link between the selectin- and the integrin-mediated lymphocyte adhesion systems

Glycobiology
P J GreenK C Nicolaou

Abstract

This communication is concerned with the binding specificity of the leukocyte-adhesion molecule L-selectin (leukocyte homing receptor) towards structurally defined sulphated oligosaccharides of the blood group Le(a) and Le(x) series, and of the glycosaminoglycan series heparin, chondroitin sulphate and keratan sulphate. The recombinant soluble form of the rat L-selectin (L-selectin-IgG Fc chimera) investigated here was shown previously to bind to lipid-linked oligosaccharides 3-O, 4-O and 6-O sulphated at galactose, such as sulphatides and a mixture of 3-sulphated Le(a)/Le(x) type tetrasaccharides isolated from ovarian cystadenoma, as well as to the HNK-1 glycolipid with 3-O sulphated glucuronic acid. In the present study, the L-selectin investigated in both chromatogram binding and plastic microwell binding experiments using neoglycolipids was found to bind to the individual 3-sulphated Le(a) and Le(x) sequences (penta-, tetra- and trisaccharides), and with somewhat lower intensities to their non-fucosylated analogues. Glycosaminoglycan disaccharides of keratan sulphate, heparin and chondroitin sulphate types were also bound by L-selectin in one or both assay systems, leading to the conclusion that clustered glycosaminoglycan ...Continue Reading

Citations

Apr 25, 1997·Journal of Immunological Methods·C R BertozziS D Rosen
Jun 26, 1998·Progress in Lipid Research·I Ishizuka
Feb 14, 2002·Resuscitation·Timothy H Rainer
Oct 1, 1996·Current Opinion in Structural Biology·P R Crocker, T Feizi
Nov 15, 2001·Advances in Anatomic Pathology·J J ListinskyG P Siegal
Oct 20, 2004·Angewandte Chemie·Sarah R HansonChi-Huey Wong
Feb 20, 1999·The Journal of Biological Chemistry·W J SandersL L Kiessling
Aug 12, 2000·The Journal of Biological Chemistry·R E BruehlS D Rosen
Aug 5, 1998·The American Journal of Physiology·R HaywardA M Lefer
Sep 29, 1999·Trends in Biochemical Sciences·T Feizi, C Galustian
Sep 2, 2005·Journal of Medicinal Chemistry·K C Nicolaou

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