PMID: 5849237Oct 1, 1965Paper

Further studies of the stimulation of DNA synthesis in cultures of spleen cell suspensions by homologous cells in inbred strains of mice and rats

The Journal of Experimental Medicine
R W Dutton

Abstract

The early proliferative response previously demonstrated in rabbits has now been shown to follow the mixing of spleen cell suspensions from 2 inbred strains of mice or rats. The size of the response is comparable to that seen in cells from hyperimmune animals exposed to antigen in vitro. Autoradiographs of cells from stimulated cultures showed 1 to 4 per cent of the total population had incorporated thymidine. Modifications in the conditions necessary for the culture of mice and rat spleen cell suspensions and the measurement of thymidine incorporation are described. No responses were observed in isologous mixes. The responses obtained on mixing individual pairs of spleens from different strains showed relatively little variation. Responses were obtained in all of the 21 possible combinations between 7 inbred strains of mice. Responses were obtained when parental cells were mixed with their F1 hybrids. Analysis of these responses showed that, in every case, parental-F1 hybrid responses were less intense than those between the 2 parents. It was shown that there was no inherent defect in the ability of the hybrid cells to respond when mixed with an unrelated strain. The results suggested that the hybrid cells made no response to ...Continue Reading

References

Jan 1, 1963·Annual Review of Microbiology·R E BILLINGHAM, W K SILVERS

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Citations

Nov 1, 1967·The American Journal of Anatomy·M R Schwarz
Jul 1, 1975·In Vitro·L A Manson, J C Palmer
Apr 27, 1984·Journal of Immunological Methods·J Barrington-Leigh
Jul 20, 1968·Nature·G A HarrisonM A Ritter
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S KanoM L Howe
Jan 1, 1972·Transplantation Reviews·F H BachG Sundharadas
Jan 1, 1973·Transplantation Reviews·P Démant
Dec 1, 1968·The Journal of Surgical Research·R G ShorterG A Hallenbeck
Jan 1, 1981·Immunological Reviews·J KornbluthB Dupont
Jan 1, 1983·Immunological Reviews·S L Swain
Aug 3, 1976·Differentiation; Research in Biological Diversity·I Olsen, G Harris
Sep 13, 1966·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·L Brent, P Medawar
Apr 1, 1966·Journal of Cellular Physiology·W T Weber
Jul 1, 1983·Cell Biochemistry and Function·I OlsenC Hand
Apr 1, 1986·European Journal of Immunology·C N BaxevanisM Papamichail
Jan 1, 1982·European Journal of Immunology·G H SunshineA A Czitrom
Jul 1, 1973·European Journal of Immunology·E Katz-HeberR E Click

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