PMID: 6168613Jan 1, 1981Paper

Further studies on the cytochemistry of the standardized silver staining of interphase nucleoli in smear preparations of Yoshida ascitic sarcoma cells in rats

Histochemistry
Z Likovský, K Smetana

Abstract

Silver staining procedure for the selective demonstration of nucleolar silver stained granules (SSG) and for the simultaneous demonstration of SSG and nucleolar silver stained matrix (SSM) were studied in smears of rat Yoshida sarcoma cells. The successful results of these procedures depend mainly on the quality of silver nitrate and formaldehyde. However, both chemicals can be easily standardized and stabilized disregarding their origin and batch. In standardized procedures (one-step procedure for the selective demonstration of SSG and two-steps procedure for the simultaneous demonstration of SSG and SSM) the silver is apparently bound to acidic groups of proteins of SSG and SSM. The proteins of SSG and SSM seem to be different but both belong to the group of acidic non-histone proteins. According to the results of digestion experiments a possibility also exists that the acidic proteins of SSG may be associated with DNA. The identification of SSG visualized by described standardized procedures was determined not only by cytochemical extraction tests but also by biological experiments. The latter demonstrated that the number of SSG in Yoshida sarcoma cells decreases after treatment of experimental animals with actinomycin D and...Continue Reading

References

Aug 20, 1979·Life Sciences·M A LischweH Busch
Jul 11, 1979·Histochemistry·K SmetanaH Busch
Nov 1, 1979·Naunyn-Schmiedeberg's Archives of Pharmacology·B Keller, K H Graefe
May 1, 1970·Experimental Cell Research·K KoshibaH Busch
Feb 15, 1980·Experientia·H KlingG Sawatzki
Jan 1, 1980·Cytogenetics and Cell Genetics·F E ArrighiA Spallone
Jan 1, 1980·Cell and Tissue Research·F WachtlerH G Schwarzacher
May 1, 1954·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C J TANDLER

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Citations

Jul 1, 1987·Cell and Tissue Research·K SmetanaH Busch
Jan 1, 1984·Cell and Tissue Research·K Smetana, Z Likovský
May 1, 1985·The Histochemical Journal·K SmetanaH Busch
Sep 6, 2005·Histochemistry and Cell Biology·Karel SmetanaZbynek Hrkal
May 1, 1984·Experimental Cell Research·K SmetanaH Busch
Jan 1, 1995·Life Sciences·Z Likovský, K Smetana
Aug 1, 1988·Cell Differentiation·V Y BrodskyE A Braga
Jan 1, 1990·Biology of the Cell·P HozákZ Likovský
Jan 1, 1997·Life Sciences·K SmetanaH Busch
May 3, 2001·Journal of Photochemistry and Photobiology. B, Biology·K SmetanaZ Hrkal
Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·K SmetanaH Busch
Mar 1, 1984·Experimental Cell Research·K SmetanaH Busch
Feb 11, 2000·Toxicologic Pathology·R C Switzer
Jul 1, 1991·Molecular Reproduction and Development·R M AmikuraH Nagano
May 1, 1987·International Journal of Cell Cloning·D D Von Hoff

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