Further studies on the identification of the phagocytosis receptor of rat retinal pigment epithelial cells

Experimental Eye Research
M O HallC Y Gregory

Abstract

We have previously produced a polyclonal antiserum (R1S5) against a plasma membrane-enriched fraction of rat retinal pigment epithelial (RPE) cells which inhibits the phagocytosis of photoreceptor outer segments (OS) by these cells. This antiserum has now been used to purify a subset of RPE membrane glycoproteins. Using a combination of lectin affinity chromatography, and chromatography on an affinity column made with R1S5-IgG, we have enriched an RPE membrane extract about 100-fold. This enriched extract contains only 12 components, all of which are glycoproteins, and retains the ability to adsorb out the inhibitory activity of antiserum R1S5. This shows that one or more of these glycoproteins recognizes an inhibitory IgG in R1S5 and suggests that one or more of these glycoproteins may participate in the phagocytosis of OS by RPE cells, possibly as the phagocytosis receptor. We have performed N-terminal microsequencing of seven of these glycoproteins: four of the seven, with Mrs of 34, 36, 51 and 55 kDa, show no sequence homology to any known proteins.

Citations

Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Silvia C FinnemannEnrique Rodriguez-Boulan
Nov 25, 1997·Proceedings of the National Academy of Sciences of the United States of America·S C FinnemannE Rodriguez-Boulan
May 1, 1999·Alternatives to Laboratory Animals : ATLA·H TähtiT Toimela
Nov 24, 2018·Experimental Eye Research·Nina Buus SørensenJens Folke Kiilgaard
Oct 3, 1999·Experimental Eye Research·S D WiltB J McLaughlin
Jul 11, 1998·Experimental Eye Research·F YanB J McLaughlin

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