PMID: 377282Apr 1, 1979Paper

Further studies on the mode of action of the heme-controlled translational inhibitor

Proceedings of the National Academy of Sciences of the United States of America
C de Haro, S Ochoa

Abstract

We have isolated [de Haro, C. & Ochoa, S. (1978) Proc. Natl. Acad. Sci. USA 75, 2713-2716] a protein factor (eIF-2 stimulating protein, ESP) that is essential for formation of ternary and 40S initiation complexes by the eukaryotic polypeptide chain initiation factor 2 (eIF-2) at the low concentrations of eIF-2 present in reticulocyte lysates. The fact that stimulation of complex formation by ESP is virtually abolished when the small (38,000 daltons) subunit of eIF-2 is phosphorylated by ATP in the presence of eIF-2 kinase (heme-controlled inhibitor, HCI) is consistent with the notion that HCI inhibits translation in lysates by blocking the interaction of eIF-2 with ESP. Our present work, with highly purified eIF-2 and ESP, has additionally established that, unlike phosphorylation of the small subunit, phosphorylation of the middle (52,000 daltons) subunit of eIF-2, which does not lead to translational inhibition in lysates, does not affect eIF-2-ESP interaction. This provides further support for our model of translational inhibition by HCI.

References

May 1, 1975·Biochimica Et Biophysica Acta·G M Walton, G N Gill
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A DattaS Ochoa
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R BenneJ W Hershey
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H Trachsel, T Staehelin
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C de HaroS Ochoa
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S M TaharaW C Merrick
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Levin, I M London
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C de Haro, S Ochoa
Jan 24, 1977·Biochemical and Biophysical Research Communications·M Gross, J Mendelewski
Oct 1, 1976·Archives of Biochemistry and Biophysics·I Harbitz, J G Hauge
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R Benne, J W Hershey

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Citations

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C de Haro, S Ochoa
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H Grosfeld, S Ochoa
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J SiekierkaS Ochoa
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K MitsuiS Ochoa
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C de HaroS Ochoa
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J SiekierkaS Ochoa
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C De HaroS Ochoa
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J SiekierkaS Ochoa
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C de HaroS Ochoa
Jun 1, 1983·Archives of Biochemistry and Biophysics·S Ochoa
Aug 14, 1987·Biochemical and Biophysical Research Communications·V Felipo, S Grisolía
May 28, 1980·Molecular and Cellular Biochemistry·M Gross
May 9, 2014·Biochemistry·Andrew M BogoradAssen Marintchev

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