Further studies on the reactions of phenylglyoxal and related reagents with proteins

Journal of Biochemistry
K Takahashi

Abstract

1. The reactivities of phenylglyoxal (PGO), glyoxal (GO), and/or methylglyoxal (MGO) with several proteins, including ribonuclease A [EC 3.1.4.22] and its derivatives, alpha-chymotrypsin [EC 3.4.21.1], trypsin [EC 3.4.21.4], lysozyme [EC 3.2.1.17], pepsin [EC 3.4.23.1], rennin [EC 3.4.23.4], thermolysin, and insulin and its B chain, have been examined. From analyses of the reaction products, PGO was shown to be the most specific for arginine residues. GO and MGO also reacted rapidly with arginine residues, but they also reacted with lysine residues to a significant extent. A side reaction with N-terminal alpha-amino groups was observed with each of these reagents. 2. Two arginine residues out of four in ribonuclease A, two out of three in alpha-chymotrypsin, one out of two in trypsin, one out of two in pepsin, and one out of five in rennin appeared to react with PGO fairly rapidly, indicating a difference in the relative accessibility of these residues by the reagent. Extensive modification of the arginine residues by PGO occurred with RCM-derivatives of ribonuclease A and insulin B chain. The N-terminal isoleucine residues of alpha-chymotrypsin and trypsin appeared to be unreactive with PGO because of salt bridge formation wit...Continue Reading

Citations

Jul 1, 1978·Die Naturwissenschaften·F Schneider
Oct 3, 1983·FEBS Letters·C Cannella, R Berni
Feb 1, 1991·Journal of Protein Chemistry·T Sejlitz, H Y Neujahr
Feb 19, 2002·Chemical & Pharmaceutical Bulletin·Yukio NoharaMitsuo Watanabe
Oct 29, 2010·Amino Acids·Naila Rabbani, Paul J Thornalley
Aug 11, 1980·FEBS Letters·G LeonciniA Bonsignore
Mar 18, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Nicolas ChabotPascal Hoffmann
Jul 20, 1999·Chemical Research in Toxicology·G P FergusonY Al-Abed
Aug 1, 1997·Archives of Biochemistry and Biophysics·I N ShipanovaR H Nagaraj
Feb 16, 2002·Biochemical and Biophysical Research Communications·William A IrwinJohn A Thomas
Jan 25, 1995·Biochimica Et Biophysica Acta·M L Riley, J J Harding
Jul 1, 1994·Toxicology Letters·M P Kalapos
Mar 28, 1986·Biochimica Et Biophysica Acta·T TamuraH Shiokawa
Jan 26, 1983·Biochimica Et Biophysica Acta·C S Schasteen, D J Reed
Sep 27, 1990·Biochimica Et Biophysica Acta·K Suzuki, K Ohnishi
Aug 13, 1981·Biochimica Et Biophysica Acta·G G Chang, T M Huang
Feb 4, 1982·Biochimica Et Biophysica Acta·V Boggaram, B Mannervik
Oct 23, 1984·Biochimica Et Biophysica Acta·I V KosarevV P Zav'yalov

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