Fusarium culmorum affects expression of biofilm formation key genes in Bacillus subtilis

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Maryam KhezriMasoud Ahmadzadeh

Abstract

It is known that there is correlation between biofilm formation and antagonistic activities of Bacillus subtilis strains; but, the mechanism of this correlation is not clear. So, the effect of the plant pathogen (Fusarium culmorum) on the biofilm formation in a B. subtilis strain with high antagonistic and biofilm formation activities was studied. The expression of sinR and tasA genes involved in the biofilm formation was studied in both single culture of bacterium (B) and co-culture with F. culmorum (FB) using real-time PCR. The results revealed that the expression of the sinR gene in both B and FB conditions was continuously decreased during the biofilm formation period and, after 24h (B4 and FB4), it reached 1% and 0.3% at the planktonic phase (B1), respectively, whereas the expression of the tasA was continuously increased and was 5.27 and 30 times more than that at the planktonic phase (B1) after 24h, respectively. So, the expression reduction rate for sinR (3 times) and the expression increasing rate for tasA (6 times) were significantly higher in FB conditions than the B ones. The relative expression of sinR in FB1 (planktonic phase), FB2 (8h), FB3(12h), and FB4 (24h) times was 0.65, 0.44, 0.35, and 0.29, whereas the tas...Continue Reading

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Citations

Apr 14, 2017·Journal of Basic Microbiology·Kulandaivelu VelmourouganeAnil Kumar Saxena
Sep 7, 2018·Mycoses·Rosa Paulina Calvillo-MedinaVictor Manuel Bautista-de Lucio
Jul 14, 2020·Frontiers in Microbiology·Sofija AndrićMarc Ongena
Jun 28, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Li LiZhen Li
Apr 8, 2021·Nature Reviews. Microbiology·Sofia ArnaouteliÁkos T Kovács

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Datasets Mentioned

BETA
HQ234328

Methods Mentioned

BETA
PCR
environmental stress

Software Mentioned

Bio
Oligo
Oligo Analyzer
SAS
Rad

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