PMID: 9636307Dec 1, 1995Paper

Fusarium graminearum A 3/5 as a novel host for heterologous protein production

Bio/technology
J C RoyerW T Yoder

Abstract

We describe a novel fungal expression system which utilizes the Quorn myco-protein fungus Fusarium graminearum A 3/5. A transformation system was developed for F. graminearum and was used to introduce the coding and regulatory regions of a trypsin gene from Fusarium oxysporum. The protein was efficiently expressed, processed and secreted by the recombinant host strain. In addition, the promoter and terminator of the F. oxysporum trypsin gene have been successfully utilized to drive the expression of a cellulase gene from Scytalidium thermophilum and a lipase gene from Thermomyces lanuginosus in F. graminearum.

References

Oct 1, 1991·Bio/technology·N S Dunn-ColemanR LaCost
Nov 1, 1991·Molecular and Cellular Biology·C E Dowzer, J M Kelly
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y H Fu, G A Marzluf
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Dec 1, 1983·Gene·J TilburnR W Davies
Jun 1, 1993·Protein Engineering·W R RypniewskiK S Wilson
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S J Vollmer, C Yanofsky

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Citations

Apr 4, 2000·Biotechnology and Bioengineering·M G WiebeA P Trinci
Apr 28, 2010·Applied Microbiology and Biotechnology·Orquídea RibeiroMerja Penttilä
Oct 3, 2013·Applied Microbiology and Biotechnology·Ulrich Kück, Julia Böhm
May 17, 2007·Biotechnology Letters·Jacob Dam SørensenMarilyn G Wiebe
Jul 19, 2000·Biochimica Et Biophysica Acta·A M BlinkovskyA V Klotz
Sep 22, 1998·Nature Biotechnology·M J de GrootA G Beijersbergen
Feb 17, 2001·European Journal of Biochemistry·F XuS H Brown
Oct 24, 2008·Phytopathology·R L Bowden, J F Leslie
Aug 9, 2013·AMB Express·Birgit JovanovićAstrid R Mach-Aigner
Apr 22, 2005·The Plant Journal : for Cell and Molecular Biology·Christian A VoigtSiegfried Salomon
Apr 26, 2000·Fungal Genetics and Biology : FG & B·D S YaverH Brody
Jun 14, 2006·Regulatory Toxicology and Pharmacology : RTP·Zofia S Olempska-BeerMichael J DiNovi
Jun 22, 2005·Trends in Biotechnology·K M Helena NevalainenPeter L Bergquist

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