Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics

Plant Biotechnology Journal
Yashwant KumarVidya S Gupta

Abstract

Molecular changes elicited by plants in response to fungal attack and how this affects plant-pathogen interaction, including susceptibility or resistance, remain elusive. We studied the dynamics in root metabolism during compatible and incompatible interactions between chickpea and Fusarium oxysporum f. sp. ciceri (Foc), using quantitative label-free proteomics and NMR-based metabolomics. Results demonstrated differential expression of proteins and metabolites upon Foc inoculations in the resistant plants compared with the susceptible ones. Additionally, expression analysis of candidate genes supported the proteomic and metabolic variations in the chickpea roots upon Foc inoculation. In particular, we found that the resistant plants revealed significant increase in the carbon and nitrogen metabolism; generation of reactive oxygen species (ROS), lignification and phytoalexins. The levels of some of the pathogenesis-related proteins were significantly higher upon Foc inoculation in the resistant plant. Interestingly, results also exhibited the crucial role of altered Yang cycle, which contributed in different methylation reactions and unfolded protein response in the chickpea roots against Foc. Overall, the observed modulations i...Continue Reading

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Citations

May 18, 2016·Trends in Plant Science·Hezi Tenenboim, Yariv Brotman
Mar 10, 2017·Plant Biotechnology Journal·Caixiang LiuHuiru Tang
Sep 30, 2018·Planta·Poonam Kanwar, Gopaljee Jha
Mar 15, 2020·International Journal of Molecular Sciences·Limiao JiangPrakash P Kumar
Aug 22, 2017·Frontiers in Plant Science·Rakesh KumarAnirudh Kumar
Mar 3, 2018·Frontiers in Plant Science·Jia LiuYongsheng Liu
Aug 25, 2019·Metabolites·Fangfang ChenXiao-Lin Chen
Nov 7, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xiaona YuSu Ryun Choi
Jun 27, 2019·Journal of Proteome Research·Caixiang LiuHuiru Tang
Jan 6, 2021·International Journal of Molecular Sciences·Marouane BaslamTakuji Ohyama
Nov 17, 2020·Frontiers in Genetics·Uday Chand JhaSwarup Kumar Parida
Oct 8, 2021·The Plant Genome·Ana Margarida SampaioMaria Carlota Vaz Patto

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Methods Mentioned

BETA
NMR
PCA
PCR
transgenic
protein folding
deamination

Software Mentioned

SIMCA
SplineCluster
Primer Express
P +
BiNGO
mlevgpphw5
AMIX
OPLS
DA
Blast2GO

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