PMID: 9420208Jan 7, 1998Paper

Fusion activity of transmembrane and cytoplasmic domain chimeras of the influenza virus glycoprotein hemagglutinin

Journal of Virology
B Schroth-DiezA Herrmann

Abstract

The role of the sequence of transmembrane and cytoplasmic/intraviral domains of influenza virus hemagglutinin (HA, subtype H7) for HA-mediated membrane fusion was explored. To analyze the influence of the two domains on the fusogenic properties of HA, we designed HA-chimeras in which the cytoplasmic tail and/or transmembrane domain of HA was replaced with the corresponding domains of the fusogenic glycoprotein F of Sendai virus. These chimeras, as well as constructs of HA in which the cytoplasmic tail was replaced by peptides of human neurofibromin type 1 (NF1) or c-Raf-1, NF78 (residues 1441 to 1518), and Raf81 (residues 51 to 131), respectively, were expressed in CV-1 cells by using the vaccinia virus-T7 polymerase transient-expression system. Wild-type and chimeric HA were cleaved properly into two subunits and expressed as trimers. Membrane fusion between CV-1 cells and bound human erythrocytes (RBCs) mediated by parental or chimeric HA proteins was studied by a lipid-mixing assay with the lipid-like fluorophore octadecyl rhodamine B chloride (R18). No profound differences in either extent or kinetics could be observed. After the pH was lowered, the above proteins also induced a flow of the aqueous fluorophore calcein from ...Continue Reading

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Citations

Oct 7, 2003·Annual Review of Biochemistry·Leonid V Chernomordik, Michael M Kozlov
Mar 15, 2005·Molecular Membrane Biology·David J Schibli, Winfried Weissenhorn
Jul 5, 2012·PloS One·Herre Jelger RisseladaMarcus Müller
Nov 2, 2013·The Journal of Biological Chemistry·Everett Clinton SmithRebecca Ellis Dutch
Jul 2, 2020·Viruses·Chelsea T Barrett, Rebecca Ellis Dutch
Jul 22, 2019·Biophysical Journal·Robert J RawlePeter M Kasson
Mar 23, 2000·Journal of Colloid and Interface Science·A Ramakrishnan, A Sadana
Oct 24, 2000·Current Opinion in Structural Biology·B R LentzK Evans
Dec 8, 2017·Journal of Virology·Julie Huiyuan XiaoAlain Townsend
Jun 26, 2001·Biophysical Journal·D V ZhelevD Needham

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