PMID: 8972702Dec 4, 1996Paper

Fusion between Jurkat cell and PEO-lipid modified liposome

Biochimica Et Biophysica Acta
N HigashiJ Sunamoto

Abstract

Direct fusion between Jurkat cell and a liposome modified with poly(ethylene oxide)-bearing lipid (PEO-lipid) was examined using diphtheria toxin fragment A (DTA) as the probe. Only the DTA-loaded liposome modified with PEO-lipid(n = 32) (n is the number of ethylene oxide units) exerted significant cytotoxicity against Jurkat cells, while liposomes lacking either the PEO-lipid or DTA did not. Liposomes modified by the PEO-lipid with shorter PEO chain(n = 5 or 15) did not show any cytotoxicity, irrespective of their DTA-loading. The cytotoxicity was observed even in the presence of cytochalasin B, an inhibitor of endocytosis. Judging from these results, we concluded that the PEO-lipid(n = 32)-modified liposome directly fused with plasma membrane of Jurkat cell.

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Citations

Aug 5, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·K KonoT Takagishi
Aug 15, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tae Woo KimSeo Young Jeong
Mar 4, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·D LetourneurM Cansell
Dec 31, 2014·International Journal of Molecular Sciences·Huei Leng Helena NgZhijun Yang
Jul 9, 1999·Journal of Biomedical Materials Research·M CansellD Letourneur
Feb 27, 2003·Journal of Liposome Research·Christopher TurnerMalcolm N Jones
Jan 10, 2003·Journal of Liposome Research·Christopher TurnerMalcolm N Jones
Dec 16, 2003·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Iren ConstantinescuMaria Gyongyossy-Issa

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