Fusion between retinal rod outer segment membranes and model membranes: a role for photoreceptor peripherin/rds

Biochemistry
Kathleen Boesze-BattagliaY Guo

Abstract

Peripherin/rds plays an essential role in the maintenance of photoreceptor rod cell disk membrane structure. The purification of this protein to homogeneity [Boesze-Battaglia, K., et al. (1997) Biochemistry 36, 6835-6846] has allowed us to characterize the functional role of peripherin/rds in the maintenance of rod outer segment (ROS) membrane fusion processes. Utilizing a cell-free fusion assay system, we report that the fusion of R18-labeled ROS plasma membrane (R18-PM) with disk membranes or peripherin/rds-enriched large unilammellar vesicles (LUVs) is inhibited upon trypsinolysis of peripherin/rds. To understand this phenomenon, we tested the ability of a series of overlapping synthetic C-terminal peripherin/rds peptides to mediate model membrane fusion. Within the 63 amino acid long region of the C-terminus, we identified a minimal 15 residue long amino acid sequence (PP-5), which is necessary to promote membrane fusion. PP-5 was able to inhibit R18-PM disk membrane fusion and promoted ANTS/DPX contents mixing in a pure vesicle system. This peptide (PP-5) promoted calcium-induced vesicle aggregation of phosphatidylethanolamine:phosphatidylserine LUVs. FTIR analysis confirmed the structural prediction of this peptide as alp...Continue Reading

References

Jan 1, 1990·Annual Review of Physiology·J M White
Jan 1, 1986·Annual Review of Neuroscience·L Stryer
Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·T StegmannA Helenius
Nov 1, 1988·The Journal of General Virology·R W RuigrokD C Wiley
Aug 1, 1988·The Journal of General Virology·S A WhartonD C Wiley
Mar 20, 1984·The Journal of Comparative Neurology·H G Jansen, S Sanyal
Apr 1, 1980·The Journal of Comparative Neurology·R H SteinbergD H Anderson
Jan 1, 1981·Neuroscience Letters·S Sanyal, H G Jansen
Mar 4, 1993·Nature·K Davies
Jan 1, 1996·Annual Review of Cell and Developmental Biology·L D HernandezJ M White

❮ Previous
Next ❯

Citations

Jun 10, 2011·Cellular and Molecular Life Sciences : CMLS·Shannon M ConleyMuna I Naash
Sep 3, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Arne M NystuenNeena B Haider
Jun 1, 2005·Progress in Lipid Research·Arlene D Albert, Kathleen Boesze-Battaglia
Jun 17, 2000·Progress in Lipid Research·N M GiustoM G Ilincheta de Boschero
Jul 10, 2004·AIDS Research and Human Retroviruses·Zhiwei WuDaniel Malamud
Jun 27, 2006·AIDS Research and Human Retroviruses·Zhiwei WuDaniel Malamud
Feb 10, 2004·Molecular Biology of the Cell·Beatrice M TamDavid S Papermaster
May 27, 2003·Investigative Ophthalmology & Visual Science·Chibo LiMuna I Naash
Aug 5, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Linda M RitterAndrew F X Goldberg
Jan 17, 2016·Progress in Retinal and Eye Research·Michael W StuckMuna I Naash
Mar 11, 2008·Progress in Retinal and Eye Research·Camiel J F BoonJan E E Keunen
Oct 24, 2006·Experimental Eye Research·Kathleen Boesze-BattagliaSusan Muller-Weeks
Apr 9, 2013·Progress in Retinal and Eye Research·Jillian N PearringVadim Y Arshavsky
Jul 26, 2002·Experimental Eye Research·Kathleen Boesze-Battagliaa, Frank P Stefano
Jul 26, 2002·Experimental Eye Research·Susan Muller-WeeksCatherine Fitzgerald
Mar 29, 2011·Experimental Eye Research·Thomas C EdringtonKathleen Boesze-Battaglia
Aug 31, 2002·Biophysical Journal·Chunhe ChenYiannis Koutalos
Dec 20, 2014·Biochimica Et Biophysica Acta·Yu-ichi OnoChiaki Nishimura
Nov 14, 2006·International Review of Cytology·Andrew F X Goldberg
Apr 12, 2001·The Journal of Biological Chemistry·C J LoewenR S Molday
May 10, 2000·Nature Medicine·A F Wright
Jun 25, 2010·Journal of Biomedical Materials Research. Part a·D JovanovicM C Harmsen
Jul 29, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Haohuai LiuYubao Li
Mar 6, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Loshini S DandeniyagePathiraja A Gunatillake
Nov 11, 2005·The Journal of Biological Chemistry·Renu Batra-SafferlingU Benjamin Kaupp
Aug 12, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mashal KakakhelMuna I Naash
Apr 5, 2000·The Journal of Biological Chemistry·J D WrigleyJ B Findlay
Nov 8, 2013·The Journal of Biological Chemistry·Brian M KevanyKrzysztof Palczewski
Mar 18, 2017·The Journal of Biological Chemistry·Juan Reyes-RevelesKathleen Boesze-Battaglia
Oct 3, 1999·The Journal of Biological Chemistry·W KedzierskiG H Travis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.