PMID: 16507248Mar 2, 2006Paper

Fusion expression, purification and bioactivity detection of human defensinalpha (HDalpha)

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Hai-yan FuZhong-liang Zhao

Abstract

To obtain recombinant human defensin alpha(HDalpha) and detect its biological activity, so as to facilitate further research. The HDalpha gene fragment with hydroxylamine cleavage site was synthesized, and then cloned into pBV220-IL-4 vector to construct pBV220-IL-4-HDalpha. The constructed vector which was confirmed to be correct by sequencing was transformed into E.coli DH5alpha and the IL-4-HDalpha fusion protein was expressed under temperature induction. After fusion protein was cleaved to remove IL-4 by hydroxylamine, purification and renaturation was performed. HDalpha's characteristics were identified by SDS-PAGE and bioactivity detection. After temperature induction, the expressed fusion protein which accounted for about 20% of total bacterial protein existed mainly in the form of inclusion body. After cleaving by hydroxylamine, the purity of obtained HDalpha was about 99.8%. Bacteriostatic test and clone forming test showed that recombinant HDalpha could obviously inhibit the growth of bacteria. The recombinant expression plasmid for HDalpha gene has been constructed successfully and obtained engineering bacteria can stably express target protein. Furthermore, techniques of purification and renaturation was set out, wh...Continue Reading

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