PMID: 10444Oct 20, 1976

Fusion in phospholipid spherical membranes. II. Effect of cholesterol, divalent ions and pH

The Journal of Membrane Biology
W Breisblatt, S Ohki

Abstract

Effect of cholesterol, divalent ions and pH on spherical bilayer membrane fusion was studied as a function of increasing temperature. Spherical bilayer membranes were composed of natural [phosphatidylcholine (PC) and phosphatidylserine (PS)] as well as synthetic (dipalmitoyl-PC, dimyristoyl-PC and dioleoyl-PC) phospholipids. Incorporation of cholesterol into the membrane (33% by weight) suppressed the fusion temperature and also greatly reduced the percentage of membrane fusion. The presence of 1 mM divalent ions (Ca++, Mg++ or Mn++) on both sides or one side of the PC membrane did not affect appreciably its fusion characteristic with temperature, but the PS membrane fusion with temperature was greatly enhanced by the presence of divalent ions. The variation of pH of the environmental solution in the range of 5.5 approximately 7.0 did not affect the membrane fusion characteristic. However, at pH 8.5, the fusion with respect to temperature was shifted toward the lower temperature by approximately 3degreesC for PC and PS membranes, and at pH 3.0 the opposite situation was observed as the fusion temperature was increased by 6degreesC for PS membranes and by 4degreesC for PC membranes The results seem to indicate that membrane flui...Continue Reading

References

Jul 3, 1975·Biochimica Et Biophysica Acta·D PapahadjopoulosG Poste
Jan 1, 1975·The Journal of Membrane Biology·W Breisblatt, S Oki
Dec 24, 1974·Biochimica Et Biophysica Acta·E Neher
Jul 1, 1971·Journal of Theoretical Biology·G Poste, A C Allison
Nov 1, 1966·Experimental Cell Research·Y Okada, F Murayama
Jul 1, 1972·The Journal of General Virology·G Poste, P Reeve
Apr 18, 1973·Nature: New Biology·Q F AhkongJ A LUCY
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Träuble, H Eibl
Mar 29, 1973·Biochimica Et Biophysica Acta·T Seimiya, S Ohki
May 30, 1974·Biochimica Et Biophysica Acta·D PapahadjopoulosW J Vail
Feb 1, 1970·Journal of Colloid and Interface Science·S Oki, O Aono
Aug 22, 1970·Nature·A R PooleJ A LUCY
Aug 22, 1970·Nature·J A LUCY
Jul 1, 1969·Journal of Colloid and Interface Science·S Ohki
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Citations

May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Jun 30, 1980·The Journal of Membrane Biology·W T MasonA D Bangham
Jun 9, 1978·The Journal of Membrane Biology·M Gratzl, G Dahl
Mar 1, 1978·Journal of Cellular Physiology·B A Herman, S M Fernandez

Related Concepts

Calcium
Cations, Divalent
Epicholesterol
Hydrogen-Ion Concentration
Magnesium
Manganese
Tissue Membrane
Membranes, Artificial
Phospholipids

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