Fusion of Bacillus stearothermophilus leucine aminopeptidase II with the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase generates a chimeric enzyme with enhanced thermostability and catalytic activity

Journal of Industrial Microbiology & Biotechnology
Yu-Wen HuaLong-Liu Lin

Abstract

Bacillus stearothermophilus leucine aminopeptidase II (LAPII) was fused at its C-terminal end with the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase. The chimeric enzyme (LAPsbd), with an apparent molecular mass of approximately 61 kDa, was overexpressed in IPTG-induced Escherichia coli cells and purified to homogeneity by nickel-chelate chromatography. The purified enzyme retained LAP activity and adsorbed raw starch. LAPsbd was stable at 70 degrees C for 10 min, while the activity of wild-type enzyme was completely abolished under the same environmental condition. Compared with the wild-type enzyme, the twofold increase in the catalytic efficiency for LAPsbd was due to a 218% increase in the k(cat) value.

References

Jun 7, 1976·Biochimica Et Biophysica Acta·E StollH Zuber
Jun 1, 1992·Bioscience, Biotechnology, and Biochemistry·I ShibuyaS Hara
Sep 1, 1992·Journal of Bacteriology·Y J Nakano, H K Kuramitsu
Jul 1, 1996·FEMS Microbiology Reviews·T Gonzales, J Robert-Baudouy
Apr 1, 1997·Applied Microbiology and Biotechnology·M TerashimaS Katoh
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M B RaoV V Deshpande
Jul 13, 2002·Archives of Microbiology·Huei-Fen LoChen-Tien Chang
Sep 5, 1995·Biotechnology and Bioengineering·B K DalmiaZ L Nikolov

❮ Previous
Next ❯

Citations

Oct 19, 2011·The Journal of Biological Chemistry·Lucas F RibeiroRichard J Ward
Jan 15, 2014·Applied Microbiology and Biotechnology·Deepika Mehta, T Satyanarayana
Jan 23, 2016·Journal of Industrial Microbiology & Biotechnology·Deepak Parashar, T Satyanarayana
Sep 27, 2012·Protein Engineering, Design & Selection : PEDS·G P FurtadoR J Ward
Oct 2, 2008·Biotechnology and Bioengineering·Zhanmin FanLing Yuan
Feb 2, 2012·Chemical Communications : Chem Comm·Preyesh StephenPing-Chiang Lyu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.