PMID: 11604163Oct 18, 2001Paper

Fusion of C3d molecule with bovine rotavirus VP7 or bovine herpesvirus type 1 glycoprotein D inhibits immune responses following DNA immunization

Veterinary Immunology and Immunopathology
S SuradhatM E Baca-Estrada

Abstract

The binding of the complement C3d molecule with receptors on B cells and/or follicular dendritic cells (FDCs) influences the induction of humoral immune responses. For example, C3d fused to an antigen has been shown to have a strong adjuvant effect on antibody production. We investigated the possibility that co-expression of antigen and C3d as a fusion protein could enhance antigen-specific immune responses, following plasmid immunization. One or two copies of murine C3d-cDNA, C3d or (C3d)(2), respectively, were cloned together with bovine rotavirus (BRV) VP7 or bovine herpesvirus type 1 (BHV-1) glycoprotein D (gD) genes. All constructs contained a signal peptide that resulted in the secretion of the expressed proteins. In vitro, the characterization of the chimeric proteins indicated that both VP7 and gD retained their antigenicity and the C3d remained biologically active. However, immunization with plasmids encoding VP7-C3d chimeras did not enhance rotavirus-specific antibody responses and the frequency of BRV-specific IFN-gamma secreting cells in the spleens were significantly lower in mice immunized with pVP7-(C3d)(2) when compared with mice immunized with plasmid encoding VP7. The same pattern of immune responses was obser...Continue Reading

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Citations

Apr 8, 2004·Vaccine·Franklin R Toapanta, Ted M Ross
May 20, 2004·Vaccine·Nadia TerrazziniTorben Lund
Aug 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Patrick P NgRonald Levy
Nov 18, 2008·Research in Veterinary Science·Guo-Xin LiGuang-Zhi Tong
Aug 15, 2006·Veterinary Immunology and Immunopathology·Matthew A FirthDouglas C Hodgins
Jul 22, 2006·Biochemical and Biophysical Research Communications·Tiezhu TongAizhen Guo
Jul 25, 2013·Human Vaccines & Immunotherapeutics·Jazmín García-MachorroLeticia Cedillo-Barrón
Apr 14, 2004·Critical Reviews in Clinical Laboratory Sciences·Sharmila ManojSylvia van Drunen Littel-van den Hurk
Oct 24, 2008·Phytopathology·Paolo CortesiMichael G Milgroom
Jul 9, 2004·World Journal of Gastroenterology : WJG·Li-Xin WangSi-Dong Xiong
Jan 29, 2019·Frontiers in Immunology·Yi YangJean M H van den Elsen
Dec 1, 2020·Expert Review of Vaccines·Taylor SicardJean-Philippe Julien
May 9, 2021·Acta Biochimica Et Biophysica Sinica·Seria Masole ShonyelaChunfeng Wang
Aug 27, 2021·Frontiers in Immunology·Ayla A WahidJean M H van den Elsen

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