Fusion of intestinal epithelial cells with bone marrow derived cells is dispensable for tissue homeostasis.

Scientific Reports
Joan H de JongLouis Vermeulen

Abstract

The epithelial lining of the intestine is characterized by an immense cellular turn-over ascertaining an extensive regenerative capacity. Multiple reports suggest that besides the local intestinal stem cell pool, circulating cells of bone marrow origin (BMDCs) contribute to this process by fusing with the epithelial lineage. However, the functional relevance of these observations is unknown. In the present study we employ a model system in which we cannot only detect cell fusion but also examine the functional importance of this process in vivo. Our results indicate that fusion between BMDCs and intestinal epithelial cells is an extremely rare event under physiological conditions. More importantly, by employing a system in which fusion-derived cells can be specifically deleted after extensive tissue damage, we present evidence that cell fusion is not relevant for tissue regeneration. Our data decisively demonstrates that intestinal epithelial homeostasis and regeneration is not dependent on cell fusion involving BMDCs.

References

Dec 6, 2000·Genesis : the Journal of Genetics and Development·A NovakC G Lobe
Mar 8, 2002·The New England Journal of Medicine·Martin KörblingZeev Estrov
Mar 25, 2004·The American Journal of Pathology·Alexandros SpyridonidisJürgen Finke
Jan 8, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Silvia MelgarErik Michaëlsson
Jun 16, 2005·Nature Reviews. Molecular Cell Biology·Brenda M OgleJeffrey L Platt
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Adnan Z RizviMelissa H Wong
Jun 21, 2007·Nature Immunology·Marc Schmidt-Supprian, Klaus Rajewsky
Mar 10, 2009·Cell·Laurens G van der FlierHans Clevers
May 18, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nienke A de VriesOlaf van Tellingen
Jul 24, 2010·Digestive Diseases and Sciences·Cem OnalAydin Yavuz
Sep 15, 2010·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Miguel WaterhouseAlexandros Spyridonidis
Oct 12, 2010·Science·Carlos Lopez-GarciaDouglas J Winton
Dec 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Robert K MontgomeryDavid T Breault
Jun 17, 2011·Nature·Jan Paul Medema, Louis Vermeulen
Nov 15, 2011·Science·Norifumi TakedaJonathan A Epstein
Dec 6, 2011·Nature Methods·Bon-Kyoung KooHans Clevers

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Citations

Nov 23, 2013·Science·Louis VermeulenDouglas J Winton
May 30, 2013·World Journal of Gastroenterology : WJG·Maria Ausiliatrice PuglisiAntonio Gasbarrini
Jan 15, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Wenda XuXiaozhong Guo
Jul 15, 2015·Stem Cells International·Michele BaroneAlfredo Di Leo
Apr 20, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Monika K GrudzinskaPiotr Religa
Dec 1, 2020·Frontiers in Pediatrics·Ling Ling LiauJia Xian Law
Mar 2, 2021·World Journal of Gastrointestinal Oncology·Yang ZhouHong-Wu Xin
Dec 26, 2017·Seminars in Pediatric Surgery·Natalie A DruckerTroy A Markel

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Methods Mentioned

BETA
Fluorescence
transgenic
FACS
PCR
irradiating
genotyping

Software Mentioned

GraphPad

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