Fusion of raft-like lipid bilayers operated by a membranotropic domain of the HSV-type I glycoprotein gH occurs through a cholesterol-dependent mechanism

Soft Matter
Giuseppe VitielloGerardino D'Errico

Abstract

A wealth of evidence indicates that lipid rafts are involved in the fusion of the viral lipid envelope with the target cell membrane. However, the interplay between these sterol- and sphingolipid-enriched ordered domains and viral fusion glycoproteins has not yet been clarified. In this work we investigate the molecular mechanism by which a membranotropic fragment of the glycoprotein gH of the Herpes Simplex Virus (HSV) type I (gH625) drives fusion of lipid bilayers formed by palmitoyl oleoyl phosphatidylcholine (POPC)-sphingomyelin (SM)-cholesterol (CHOL) (1 : 1 : 1 wt/wt/wt), focusing on the role played by each component. The comparative analysis of the liposome fusion assays, Dynamic Light Scattering (DLS), spectrofluorimetry, Neutron Reflectivity (NR) and Electron Spin Resonance (ESR) experiments, and Molecular Dynamics (MD) simulations shows that CHOL is fundamental for liposome fusion to occur. In detail, CHOL stabilizes the gH625-bilayer association by specific interactions with the peptide Trp residue. The interaction with gH625 causes an increased order of the lipid acyl chains, whose local rotational motion is significantly hampered. SM plays only a minor role in the process, favoring the propagation of lipid perturba...Continue Reading

References

Oct 1, 1987·The Journal of Cell Biology·G van MeerK Simons
Jul 7, 1981·Biochemistry·D K StruckR E Pagano
Mar 6, 2002·The Journal of Experimental Medicine·Sina BavariM Javad Aman
Aug 2, 2003·Molecular Membrane Biology·Satinder S RawatAnu Puri
Aug 14, 2003·Journal of Virology·Florent C BenderGary H Cohen
Jun 7, 2005·The Journal of Biological Chemistry·Stefania GaldieroMassimiliano Galdiero
Oct 14, 2005·Journal of Computational Chemistry·James C PhillipsKlaus Schulten
Aug 2, 2006·Biological & Pharmaceutical Bulletin·Takashi Suzuki, Yasuo Suzuki
Sep 1, 2006·The Journal of Cell Biology·Katharina GausMartin A Schwartz
Feb 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ravi P Subramanian, Robert J Geraghty
May 29, 2007·Biochimica Et Biophysica Acta·Jason E Cummings, T Kyle Vanderlick
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Aaron FarnsworthDavid C Johnson
Aug 28, 2007·Seminars in Cell & Developmental Biology·Neetu Gupta, Anthony L DeFranco
Jul 4, 2008·Nature Structural & Molecular Biology·Stephen C Harrison
Nov 7, 2009·Biochimica Et Biophysica Acta·Roberta SpadacciniDelia Picone
Jan 21, 2010·Biochimica Et Biophysica Acta·Stefania GaldieroMassimiliano Galdiero
Mar 10, 2010·Sub-cellular Biochemistry·Cornelia Schroeder
May 26, 2010·The Journal of Physical Chemistry. B·Jeffery B KlaudaRichard W Pastor
Jul 6, 2010·Nature Structural & Molecular Biology·Tirumala K ChowdaryEkaterina E Heldwein
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tatiana GianniGabriella Campadelli-Fiume
Jun 2, 2011·Cold Spring Harbor Perspectives in Biology·Kai Simons, Julio L Sampaio
Jul 15, 2011·Molecular & Cellular Proteomics : MCP·Yu Zi ZhengLeonard J Foster
Dec 16, 2011·Journal of Virology·Tatiana Gianni, Gabriella Campadelli-Fiume
Dec 24, 2011·Biochimica Et Biophysica Acta·Juan José MartínIsabel Muñoz-Barroso
Feb 24, 2012·Biomaterials·Gaetano MangiapiaLuigi Paduano
Jun 28, 2012·Frontiers in Physiology·David A HicksAnthony J Turner
Jul 16, 2013·The European Physical Journal. E, Soft Matter·Mitushiro HiraiGiuseppe Zaccai
Jul 31, 2013·Biochimica Et Biophysica Acta·Ariel Alcides PetrukUmberto Oreste
Oct 23, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Giuseppe VitielloGerardino D'Errico
Nov 13, 2013·Molecular Genetics and Metabolism·Anatália LabilloyOra A Weisz
Apr 30, 1998·The Journal of Physical Chemistry. B·A D MacKerellM Karplus
Oct 12, 2014·Biochimica Et Biophysica Acta·Stefania GaldieroMassimiliano Galdiero
Oct 28, 2014·The Journal of Physical Chemistry. B·Cayla M MillerJeetain Mittal

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Citations

May 8, 2016·Chemistry and Physics of Lipids·Trivikram R Molugu, Michael F Brown
Jul 19, 2016·Biochimica Et Biophysica Acta·A AgopianS Castano
Oct 30, 2015·International Journal of Molecular Sciences·Annarita FalangaStefania Galdiero
Mar 20, 2020·International Journal of Molecular Sciences·Manuela GrimaldiAnna Maria D'Ursi
Jun 13, 2018·Scientific Reports·Rosario OlivaLuigi Petraccone
Jun 24, 2020·Nanomaterials·Carlo CaianielloGerardino D'Errico
Sep 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Claudia RiccardiDaniela Montesarchio
Feb 23, 2021·Frontiers in Microbiology·Inés RipaRaquel Bello-Morales
Sep 6, 2018·Current Opinion in Virology·Jodi A Hadden, Juan R Perilla
Dec 12, 2020·Journal of Colloid and Interface Science·Alessandra LuchiniLise Arleth
Jun 3, 2021·Journal of Chemical Theory and Computation·Sebastian Franco-UlloaMarco De Vivo
Jul 3, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Alessandra LuchiniGiuseppe Vitiello
Dec 5, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Marco A DeriuAndrea Danani
Feb 14, 2019·Chemical Reviews·Valentina CorradiD Peter Tieleman

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