Fusion of simian immunodeficiency virus with liposomes and erythrocyte ghost membranes: effects of lipid composition, pH and calcium

The Journal of General Virology
C E LarsenN Düzgüneş

Abstract

Simian immunodeficiency virus from macaques (SIVmac) is closely related in its structure and biological activity to human immunodeficiency virus, and is the best animal model for the acquired immunodeficiency syndrome. We investigated the kinetics of membrane fusion between SIVmac and phospholipid vesicles and the effects of various parameters on this process. Purified SIVmac was labelled with octadecyl rhodamine B chloride, and fusion was continuously monitored as the dilution of the probe in target membranes. These studies show that SIVmac fusion is strongly dependent upon the liposome composition. Fusion with pure cardiolipin (CL) liposomes is significantly faster than with CL/dioleoylphosphatidylcholine (DOPC) (3:7), phosphatidylserine (PS) or disialoganglioside (GD1a)/DOPC (1.5:8.5) vesicles. SIVmac does not fuse appreciably with pure DOPC liposomes. Reduction of pH from 7.5 to 4.5 greatly enhances the rate of SIVmac fusion with CL, CL/DOPC and PS membranes, but does not affect fusion with DOPC or GD1a/DOPC membranes. Calcium stimulates viral fusion with CL liposomes, but not with CL/DOPC or DOPC liposomes. SIVmac fuses with human erythrocyte ghost membranes only slowly at reduced pH. Our results indicate that SIVmac can f...Continue Reading

Citations

Apr 5, 2000·Pathology Oncology Research : POR·J OngrádiM Bendinelli
Jul 15, 1996·Chemistry and Physics of Lipids·L Chernomordik
Jul 3, 2002·Chemistry and Physics of Lipids·Robert BlumenthalAnu Puri
Oct 13, 1998·Journal of Drug Targeting·A Désormeaux, M G Bergeron
Jun 1, 1997·Medical Hypotheses·A Richardson
Jul 1, 1995·The American Journal of Physiology·S KeayW F Goldman
Dec 9, 1991·Biochimica Et Biophysica Acta·M C Pedroso de LimaN Düzgüneş

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