PMID: 6163458Feb 6, 1981Paper

Fusion of small unilamellar liposomes with phospholipid planar bilayer membranes and large single-bilayer vesicles

Biochimica Et Biophysica Acta
N Düzgüneş, S Ohki

Abstract

Small unilamellar phosphatidylserine/phosphatidylcholine liposomes incubated on one side of planar phosphatidylserine bilayer membranes induced fluctuations and a sharp increase in the membrane conductance when the Ca2+ concentration was increased to a threshold of 3--5 mM in 100 mM NaCl, pH 7.4. Under the same ionic conditions, these liposomes fused with large (0.2 micrometer diameter) single-bilayer phosphatidylserine vesicles, as shown by a fluorescence assay for the mixing of internal aqueous contents of the two vesicle populations. The conductance behavior of the planar membranes was interpreted to be a consequence of the structural rearrangement of phospholipids during individual fusion events and the incorporation of domains of phosphatidylcholine into the Ca2+-complexed phosphatidylserine membrane. The small vesicles did not aggregate or fuse with one another at these Ca2+ concentrations, but fused preferentially with the phosphatidylserine membrane, analogous to simple exocytosis in biological membranes. Phosphatidylserine vesicles containing gramicidin A as a probe interacted with the planar membranes upon raising the Ca2+ concentration from 0.9 to 1.2 mM, as detected by an abrupt increase in the membrane conductance....Continue Reading

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Citations

Dec 8, 1982·Biochimica Et Biophysica Acta·F GambaleC Marchetti
Mar 3, 2009·Biochimica Et Biophysica Acta·A M BouchetE A Disalvo
Jul 22, 2014·Progress in Neurobiology·Stephen D MerineyCameron B Gundersen
Jul 25, 2012·Biophysical Journal·Alberto Martín-MolinaJordi Faraudo
Nov 12, 2009·Methods in Enzymology·Anna V Shnyrova, Joshua Zimmerberg
Jan 27, 2005·The Anatomical Record. Part B, the New Anatomist·Robert B Silver, George D Pappas
Jul 14, 1982·Biochimica Et Biophysica Acta·S Ohki

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