Fusion pore expansion is a slow, discontinuous, and Ca2+-dependent process regulating secretion from alveolar type II cells

The Journal of Cell Biology
T HallerK Maly

Abstract

In alveolar type II cells, the release of surfactant is considerably delayed after the formation of exocytotic fusion pores, suggesting that content dispersal may be limited by fusion pore diameter and subject to regulation at a postfusion level. To address this issue, we used confocal FRAP and N-(3-triethylammoniumpropyl)-4-(4-[dibutylamino]styryl) pyridinium dibromide (FM 1-43), a dye yielding intense localized fluorescence of surfactant when entering the vesicle lumen through the fusion pore (Haller, T., J. Ortmayr, F. Friedrich, H. Volkl, and P. Dietl. 1998. Proc. Natl. Acad. Sci. USA. 95:1579-1584). Thus, we have been able to monitor the dynamics of individual fusion pores up to hours in intact cells, and to calculate pore diameters using a diffusion model derived from Fick's law. After formation, fusion pores were arrested in a state impeding the release of vesicle contents, and expanded at irregular times thereafter. The expansion rate of initial pores and the probability of late expansions were increased by elevation of the cytoplasmic Ca2+ concentration. Consistently, content release correlated with the occurrence of Ca2+ oscillations in ATP-treated cells, and expanded fusion pores were detectable by EM. This study sup...Continue Reading

References

Dec 1, 1992·The Journal of Cell Biology·J R Monck, J M Fernandez
Jan 1, 1992·The Clinical Investigator·H Wirtz, M Schmidt
Jan 1, 1990·Annual Review of Physiology·W Almers
Feb 1, 1989·Journal of Applied Physiology·D WarburtonL Cosico
Nov 1, 1987·The American Journal of Physiology·K SanoR J Mason
Sep 1, 1986·The Anatomical Record·T Manabe, H Ikeda
Sep 14, 1984·Biochimica Et Biophysica Acta·W R RiceJ A Whitsett
Nov 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E C Tsilibary, M C Williams
Apr 24, 1995·FEBS Letters·J Hartmann, M Lindau
Apr 24, 1995·FEBS Letters·R Fernández-Chacón, G Alvarez de Toledo
Sep 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S A RooneyC R Mendelson
Jun 1, 1996·Current Opinion in Neurobiology·W J BetzC B Smith
Jan 1, 1997·Neuron·R Rahamimoff, J M Fernandez
Jan 1, 1997·Annual Review of Physiology·J A Clements
Aug 1, 1997·The American Journal of Physiology·L M Coluccio
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·T HallerP Dietl
Apr 29, 1998·Annual Review of Physiology·W J Betz, J K Angleson
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·E R Travis, R M Wightman
Jan 5, 1999·American Journal of Respiratory and Critical Care Medicine·F RoseF Grimminger
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y AshinoJ Bhattacharya
Nov 9, 2000·Cell Biology International·P Dietl, T Haller
Mar 7, 2001·Nature Cell Biology·T NemotoH Kasai
Jul 14, 2001·Histochemistry and Cell Biology·M W Hess, P Siljander

❮ Previous
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Citations

Jan 5, 2005·Nature Reviews. Immunology·Jo Rae Wright
Aug 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Pika MiklavcManfred Frick
Sep 26, 2008·Antioxidants & Redox Signaling·Latoya N Johnson, Michael Koval
Jan 27, 2006·Molecular Biology of the Cell·Anna M Sokac, William M Bement
Jan 19, 2007·Molecular Biology of the Cell·Tamar GattegnoBenjamin Podbilewicz
Jun 29, 2007·Molecular Biology of the Cell·Olga LarinaPeter Thorn
Jan 23, 2009·Molecular Biology of the Cell·Purnima Bhat, Peter Thorn
Jan 7, 2011·Molecular Biology of the Cell·Elisabeth T BarfodSteven D Lidofsky
Feb 16, 2005·Annual Review of Physiology·Paul Dietl, Thomas Haller
Jan 29, 2011·American Journal of Physiology. Cell Physiology·Andrea RavasioThomas Haller
May 23, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nina HobiThomas Haller
Jan 25, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Paul JenningsPaul Dietl
Jan 27, 2011·Journal of Cell Science·Amarjit MishraLin Liu
Apr 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Peter ThornIan Parker
Sep 24, 2002·Seminars in Cell & Developmental Biology·Timothy E WeaverMildred Stahlman
Sep 29, 2007·Experimental Cell Research·Matjaz StenovecRobert Zorec
Oct 21, 2015·Communicative & Integrative Biology·Kathrin Neuland, Manfred Frick
Mar 19, 2005·Journal of Neurochemistry·Flávia R CarreñoEmer S Ferro
Dec 21, 2002·Synapse·Jean Vautrin, Jeffery L Barker
Feb 4, 2006·Microscopy Research and Technique·Michael W HessLukas A Huber
Mar 27, 2012·Communicative & Integrative Biology·Pika Miklavc, Manfred Frick
Oct 20, 2006·Journal of Neuroscience Methods·Gareth John Owen Evans, Michael Alan Cousin
Jan 28, 2003·Biophysical Journal·Wolfgang SingerPaul Dietl
May 1, 2012·Trends in Cell Biology·Thomas D NightingaleLouise P Cramer
Oct 30, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Bárbara OlmedaJesus Pérez-Gil
Sep 7, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiaohui FangMichael A Matthay
May 16, 2006·Journal of Biomolecular Screening·A WemhönerT Haller
May 15, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alexandra V AndreevaTatyana A Voyno-Yasenetskaya
Aug 21, 2019·Scientific Reports·M Tabitha MüllerPika Miklavc
Dec 29, 2017·The Journal of General Physiology·Giorgio FoisManfred Frick
Aug 12, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ignacio Garcia-VerdugoThomas Haller
Nov 12, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mohammad N IslamJahar Bhattacharya
Oct 27, 2016·Cell and Tissue Research·Elena Lopez-RodriguezAnna Serrano-Mollar
Jul 18, 2020·International Journal of Molecular Sciences·Eva WirschingManfred Frick

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Methods Mentioned

BETA
fluorescence microscopy
Fluorescence
transmission electron microscopy
scanning electron microscopy
optical tweezers

Software Mentioned

DatGraf
TILLvisION
Adobe Photoshop
Excel

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