Fusion protein linkers: property, design and functionality

Advanced Drug Delivery Reviews
Xiaoying ChenWei-Chiang Shen

Abstract

As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of stable, bioactive fusion proteins. This review covers the current knowledge of fusion protein linkers and summarizes examples for their design and application. The general properties of linkers derived from naturally-occurring multi-domain proteins can be considered as the foundation in linker design. Empirical linkers designed by researchers are generally classified into 3 categories according to their structures: flexible linkers, rigid linkers, and in vivo cleavable linkers. Besides the basic role in linking the functional domains together (as in flexible and rigid linkers) or releasing the free functional domain in vivo (as in in vivo cleavable linkers), linkers may offer many other advantages for the production of fusion proteins, such as improving biological activity, increasing expression yield, and achieving desirable pharmacokinetic profiles.

References

Apr 1, 1992·Proteins·A L MorrisJ M Thornton
Nov 24, 1986·FEBS Letters·J S EvansC F Higgins
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J S HustonR Crea
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Marqusee, R L Baldwin
Jul 1, 1995·Protein Engineering·K AlfthanT T Teeri
Jan 17, 1997·The Journal of Biological Chemistry·R AuroraG D Rose
Mar 14, 1998·Trends in Neurosciences·V W YongD R Edwards
Aug 26, 1998·The American Journal of Pathology·K LampertB Volk
Jul 15, 1999·Nature Biotechnology·G S WaldoT C Terwilliger
Feb 19, 2000·Current Opinion in Chemical Biology·R S Gokhale, C Khosla
Jun 2, 2000·Protein Engineering·C J Crasto, J A Feng
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Gary Thomas
Jan 23, 2003·Protein Engineering·Richard A George, Jaap Heringa
Jun 10, 2003·Nature Structural Biology·Stefan HenrichManuel E Than
Oct 7, 2003·Nature Biotechnology·Gill AdamsYuti Chernajovsky
Dec 24, 2004·Diabetes·Alokesh DuttaroyOlivier Blondel
Feb 15, 2005·Protein Engineering, Design & Selection : PEDS·Sandrine VessillierYuti Chernajovsky
May 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yun BaiWei-Chiang Shen
Nov 22, 2005·Nature Methods·Rafael Yuste
Feb 14, 2006·Biochemical Pharmacology·Donald E Mager
May 27, 2006·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Jennifer A DumontAlan J Bitonti
Oct 10, 2006·The AAPS Journal·Robert J Kreitman
Nov 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefan WernerYuri Gleba
Apr 20, 2007·Pharmaceutical Research·Leena N PatelWei-Chiang Shen
Dec 22, 2007·Nature Reviews. Drug Discovery·Benjamin LeaderDavid E Golan
Apr 9, 2008·Thrombosis and Haemostasis·Thomas WeimerStefan Schulte
Apr 17, 2008·Applied Microbiology and Biotechnology·Ping Lu, Ming-Guang Feng

❮ Previous
Next ❯

Citations

Feb 19, 2013·Annual Review of Chemical and Biomolecular Engineering·Yen-Hsiang WangChristina D Smolke
May 3, 2014·Biomaterials·Dongwook KimRihe Liu
Jun 20, 2014·Journal of Immunological Methods·Jinny L LiuGeorge P Anderson
Oct 11, 2014·Protein Engineering, Design & Selection : PEDS·Joshua S KleinPamela J Bjorkman
Feb 19, 2016·Chemical Reviews·Elena PapaleoRuth Nussinov
Jul 22, 2014·Expert Opinion on Drug Discovery·Toshihiko SugikiChojiro Kojima
Sep 12, 2015·FEBS Letters·Jennifer S McDowallFrank Sargent
Nov 26, 2015·Chembiochem : a European Journal of Chemical Biology·Yu-Yung Chang, Chun-Hua Hsu
Jan 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·David T RodgersTravis S Young
Dec 3, 2014·Biotechnology Advances·Kai YuDong-Yup Lee
Dec 24, 2015·Protein Science : a Publication of the Protein Society·Rei MatsuokaDaisuke Kohda
Jan 11, 2016·Applied Biochemistry and Biotechnology·Shima Moradi-KalbolandiMohammad Ali Shokrgozar
Mar 20, 2016·Plant Science : an International Journal of Experimental Plant Biology·Stefan G van WykBarend J Vorster
Nov 28, 2015·Drug Discovery Today·Michael C Wehr, Moritz J Rossner
Dec 5, 2015·Frontiers in Bioengineering and Biotechnology·Anselm H C Horn, Heinrich Sticht
Jan 15, 2015·Angewandte Chemie·Tobias W GiessenMohamed A Marahiel
Jul 22, 2014·The FEBS Journal·Izabela SabalaMatthias Bochtler
May 21, 2015·Protein Engineering, Design & Selection : PEDS·Keyu LiAnna M Wu
Apr 3, 2016·Applied Microbiology and Biotechnology·Timo StresslerLutz Fischer
Mar 20, 2015·Biotechnology and Bioengineering·Christian WillrodtMattijs K Julsing
Jul 17, 2015·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·William R Strohl
Aug 20, 2015·BMC Biotechnology·Gro Elin Kjæreng Bjerga, Adele Kim Williamson
Feb 1, 2015·Applied Microbiology and Biotechnology·Eun-Yeong JeonJin-Byung Park
Mar 10, 2015·Journal of Biological Engineering·Sayed Shahabuddin Hoseini, Martin G Sauer
Feb 24, 2015·Journal of Biotechnology·Maximilian KlementDong-Yup Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here