Fusogen-mediated neuron-neuron fusion disrupts neural circuit connectivity and alters animal behavior

Proceedings of the National Academy of Sciences of the United States of America
Rosina Giordano-SantiniMassimo A Hilliard


The 100-y-old neuron doctrine from Ramón y Cajal states that neurons are individual cells, rejecting the process of cell-cell fusion in the normal development and function of the nervous system. However, fusogens-specialized molecules essential and sufficient for the fusion of cells-are expressed in the nervous system of different species under conditions of viral infection, stress, or disease. Despite these findings, whether the expression of fusogens in neurons leads to cell-cell fusion, and, if so, whether this affects neuronal fate, function, and animal behavior, has not been explored. Here, using Caenorhabditis elegans chemosensory neurons as a model system, we provide proof-of-principle that aberrant expression of fusogens in neurons results in neuron-neuron fusion and behavioral impairments. We demonstrate that fusion between chemoattractive neurons does not affect the response to odorants, whereas fusion between chemoattractive and chemorepulsive neurons compromises chemosensation. Moreover, we provide evidence that fused neurons are viable and retain their original specific neuronal fate markers. Finally, analysis of calcium transients reveals that fused neurons become electrically coupled, thereby compromising neural ...Continue Reading


Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G Fischer von MollardT C Südhof
Nov 15, 1985·Science·H M BlauC Webster
Oct 1, 1986·Developmental Biology·L A PerkinsJ G Culotti
Oct 1, 1983·The Journal of Comparative Neurology·P S Albert, D L Riddle
Nov 1, 1983·Developmental Biology·J E SulstonJ N Thomson
Aug 13, 1993·Cell·Cornelia I BargmannH R Horvitz
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·L Bloom, H R Horvitz
Jul 27, 1999·Genes & Development·A SagastiCornelia I Bargmann
May 24, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Gidi Shemer, Benjamin Podbilewicz
Mar 7, 2002·Developmental Cell·William A MohlerBenjamin Podbilewicz
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ryoko AndoAtsushi Miyawaki
Aug 28, 2003·Médecine sciences : M/S·Christelle Gally, Jean-Louis Bessereau
Sep 3, 2004·Current Biology : CB·Gidi ShemerBenjamin Podbilewicz
May 26, 2006·Annual Review of Genomics and Human Genetics·Norbert Bannert, Reinhard Kurth
Oct 3, 2006·Developmental Cell·Benjamin PodbilewiczLeonid V Chernomordik
Nov 23, 2006·Nature Neuroscience·Maulik R PatelKang Shen
May 10, 2007·Developmental Cell·Amir SapirBenjamin Podbilewicz
Nov 2, 2007·Nature·Sreekanth H ChalasaniCornelia I Bargmann
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Fabio PianoMarc Vidal
Feb 11, 2009·Genes & Development·Bluma J LeschCornelia I Bargmann
Oct 31, 2009·PLoS Pathogens·Kelly M McCarthyLynn W Enquist
Mar 6, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anindya Ghosh-RoyAndrew D Chisholm
May 8, 2010·Science·Meital Oren-SuissaBenjamin Podbilewicz
Mar 19, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brent NeumannMassimo A Hilliard
Mar 26, 2011·Science·Ori AvinoamBenjamin Podbilewicz
Nov 22, 2011·Nature Neuroscience·Tapan A ManiarCornelia I Bargmann
Nov 12, 1986·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G WhiteS Brenner
May 23, 2012·Cell Host & Microbe·Tal Kramer, Lynn W Enquist
Dec 15, 2012·Trends in Neurosciences·Andrei B Belousov, Joseph D Fontes
Aug 14, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christian LavialleThierry Heidmann
Aug 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Andrea E GranstedtLynn W Enquist
Apr 15, 2014·Cell·Jimena Pérez-VargasFélix A Rey

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