Future potential of targeted component analysis by multidimensional liquid chromatography-mass spectrometry

Journal of Chromatography. a
F Regnier, G Huang

Abstract

Multidimensional liquid chromatography (MDLC) may be used in either (i) the profiling mode where it is the objective to fractionate all components in a mixture or (ii) the targeted component mode in which it is the objective to determine specific analytes. This paper focuses on targeted component analysis from complex mixtures, addressing the critical operations of analyte selection and transport from the first to the second dimension. Although the physical operation of switching a component into the second dimension with computer controlled valving is simple, it is shown that changes in analyte retention time and peak width with column age and fouling are a serious problem. The analyte moves out of the preselected time window for valve switching and quantitation is compromised in the second dimension. It is proposed that a solution to the "drifting peak" phenomenon in targeted component analysis is to use binary mobility elution in the first dimension. Binary mobility refers to those systems, such as affinity chromatography, in which analyte mobility is generally either 0 or 1 relative to mobile phase velocity. Coupling these binary changes in analyte mobility in the first dimension with valve switching eliminates the "driftin...Continue Reading

References

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Citations

Aug 19, 2007·Microbial Cell Factories·Robert Lj GrahamGeoff McMullan
Sep 15, 2006·Journal of Separation Science·Pavel Jandera
Dec 6, 2005·Journal of Chromatography. a·Pavel JanderaLenka Kolárová
Dec 26, 2001·Chemical Reviews·S Liu, D S Edwards
Nov 10, 2000·Journal of Chromatography. a·K WagnerG Marko-Varga
Jan 19, 2000·Bioconjugate Chemistry·S LiuD S Edwards

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