PMID: 8592208Nov 1, 1995Paper

G alpha o1 decapeptide modulates the hippocampal 5-HT1A potassium current

Journal of Neurophysiology
S Oleskevich

Abstract

1. The serotonin1A (5-HT1A) receptor is coupled to an inwardly rectifying potassium current (IKir) via a G protein. The identity of the G-protein subtype was investigated with 2 10-amino acid peptides derived from the carboxyl (C) terminus of the alpha-subunits of the Go1 and Gi2 proteins (G alpha o1 and G alpha i2). The synthetic decapeptides were applied by intracellular perfusion during whole cell recording from dentate granule cells in the hippocampal slice preparation. 2. Bath application of 5-HT produced an IKir, which was blocked by the selective 5-HT1A receptor antagonist, pindobind5-HT1A. The G alpha o1 peptide inhibited the 5-HT1A IKir by 60 +/- 7% (mean +/- SE; t = 30 min), whereas the G alpha i2 peptide had no effect. The G alpha o1 peptide produced a slowly developing outward current that was not observed in the absence of peptide or in the presence of the G alpha i2 peptide. 3. The results indicate that G alpha o1 and not G alpha i2 modulates the 5-HT1A IKir in hippocampal granule cells. They also suggest that G alpha o1 occludes the 5-HT1A response by direct activation of the IKir. The intracellular perfusion of synthetic G alpha peptides provides a new approach to identify the G-protein subtype(s) in a receptor-...Continue Reading

Citations

Jun 15, 2006·Molecular Pharmacology·Clotilde Mannoury la CourLaurence Lanfumey
Jun 3, 2005·Neuroreport·Sharon OleskevichIan A Hendry
Mar 1, 2007·Current Protocols in Pharmacology·Rita Raddatz, Richard H Alper
Aug 12, 2015·International Journal of Molecular Sciences·Zdzisław ChilmonczykIngebrigt Sylte
Aug 30, 2000·Nuclear Medicine and Biology·L Lanfumey, M Hamon
Jan 16, 1998·European Journal of Pharmacology·V FabreA M Laporte
May 7, 1998·European Journal of Pharmacology·R H Alper, D L Nelson
Jan 22, 2013·ACS Chemical Neuroscience·Stefanie C AltieriAnne M Andrews
Apr 7, 2010·Cellular Signalling·Abigail M Polter, Xiaohua Li

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