Abstract
To study the early stages of development from stem cells of the CD56+ cell population [which includes natural killer (NK) cells], granulocyte-colony stimulating factor-mobilized peripheral blood CD34+ cells from healthy donors were sorted to >99% purity and cultured in the presence of stem cell factor and interleukin (IL)-2. After 3 weeks in culture, the majority of cells acquired CD33, with or without human leukocyte antigen-DR and CD14. In 20 stem cell donors tested, 8.7 +/- 8.8% of cells were CD56+. Two major CD56+ subsets were identified: CD56(bright), mainly CD33- cells (7+/-10%, n=11) with large, granular lymphocyte morphology, and CD56dim, mainly CD33+ (2.5+/-2, n=11) cells with macrophage morphology. The CD56bright population had cytoplasmic granzyme A but lacked killer inhibitory receptor, suggesting they were immature NK cells. The CD56dim, CD33+, population lacked NK markers. They may represent a minor subset of normal monocytes at a developmental stage comparable with the rare CD56+ CD33+ hybrid myeloid/NK cell leukemia. Consistent with a monocyte nature, CD56dimCD33+ proliferated and produced a variety of cytokines upon lipopolysaccharide stimulation, including IL-8, IL-6, monocyte chemoattractant protein-1, and ma...Continue Reading
References
Jun 1, 1992·Seminars in Cell Biology·C Goridis, J F Brunet
Jan 1, 1982·Cancer Immunology, Immunotherapy : CII·B MytarA Czupryna
Jan 1, 1993·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·M R SilvaJ L Ascensao
Oct 1, 1995·Immunity·A GalyB Chen
May 13, 1998·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·F VazJ L Ascensao
May 15, 1998·Journal of Leukocyte Biology·S N BykovskajaJ Shogan
Jun 30, 1998·European Journal of Immunology·G CarayolA Caignard
Apr 19, 2000·Lancet·R PowlesS Singhal
Jan 9, 2001·Experimental Hematology·L KobariL Douay
May 22, 2001·Journal of Hematotherapy & Stem Cell Research·L KobariL Douay
Nov 28, 2001·British Journal of Haematology·R NakamuraA J Barrett
Feb 28, 2002·Blood·Jean FeuillardMarie-Christine Béné
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Simona SivoriAlessandro Moretta
Jul 20, 2002·The American Journal of Surgical Pathology·Tony PetrellaLaurent Galibert
Dec 31, 2002·Blood·Sonia A PerezMichael Papamichail
Mar 19, 2003·Journal of Cutaneous Pathology·Jeff D HarvellUma Sundram
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isabel BaraoJoao L Ascensao
Citations
Dec 2, 2008·Biogerontology·Mario MuzzioliMauro Provinciali
Feb 7, 2012·Leukemia·T M WestersA A van de Loosdrecht
Oct 22, 2005·Leukemia·F GuillotonA Quillet-Mary
Jul 1, 2006·Leukemia·G SconocchiaA J Barrett
Nov 30, 2013·Arthritis Research & Therapy·Marco KrasseltManuela Rossol
Jun 24, 2009·Haematologica·Arjan A van de LoosdrechtTheresia M Westers
Feb 20, 2007·The International Journal of Biochemistry & Cell Biology·Mario MuzzioliMauro Provinciali
Aug 18, 2010·International Journal of Cancer. Journal International Du Cancer·Giuseppe SconocchiaGiulio C Spagnoli
Jan 23, 2015·Oncoimmunology·Giuseppe SconocchiaSoldano Ferrone
May 26, 2009·São Paulo Medical Journal = Revista Paulista De Medicina·Jerusa Martins CarvalhoJosé Salvador Rodrigues de Oliveira
Jun 28, 2007·Journal of Leukocyte Biology·Malgorzata StecMarek Zembala
Nov 5, 2004·Leukemia·G SconocchiaA J Barrett
Mar 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Giuseppe SconocchiaA John Barrett
Apr 16, 2005·Journal of Translational Medicine·Giuseppe SconocchiaA John Barrett