G-CSF prevents the suppression of bone marrow hematopoiesis induced by IL-12 and augments its antitumor activity in a melanoma model in mice

Annals of Oncology : Official Journal of the European Society for Medical Oncology
J GołabM Jakóbisiak

Abstract

IL-12 has been successfully used in experimental tumor therapy. However, administration of this cytokine induces dose-dependent suppression of hematopoiesis that could potentially limit its use in clinical trials. We decided to examine whether the myelosuppressive activity of IL-12 could be corrected by the administration of G-CSF. In the initial experiments the influence of IL-12 and/or G-CSF on bone marrow and spleen GM-CFC was evaluated. To examine whether C-CSF could influence the antitumor activity of IL-12 the combination therapy with these agents was carried out starting on day seven following inoculation of melanoma MmB16 cells into the footpads of B6D2F1 mice. To obtain insight into the mechanism of the observed augmented antitumor activity of the combination therapy with IL-12 and G-CSF, the influence of these cytokines on macrophage activity (cytotoxicity and nitric oxide release) was analyzed. In accord with our expectations, the application of G-CSF partially prevented the suppression of bone marrow myelopoiesis in IL-12 treated mice. Unexpectedly, G-CSF also showed potentiation of antitumor effects of IL-12 in this melanoma model. The augmented antitumor activity of combined IL-12/G-CSF immunotherapy could result ...Continue Reading

Citations

May 18, 1999·Journal of the National Cancer Institute·A DabrowskaM Jakóbisiak
Jun 15, 1999·Toxicologic Pathology·B D CarT D Anderson
Apr 14, 2006·Expert Opinion on Biological Therapy·Michael Y ShapiraShimon Slavin
Aug 10, 1999·International Journal of Dermatology·K J Smith, H Skelton
Aug 27, 2005·Japanese Journal of Clinical Oncology·Ugo BottoniStefano Calvieri
Feb 12, 2014·Cancer Immunology, Immunotherapy : CII·Witold LasekMarek Jakobisiak
Feb 22, 2002·International Journal of Cancer. Journal International Du Cancer·Adam GiermaszJakub Gołab

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.