PMID: 9419813Jan 7, 1998Paper

G-protein activation, intracellular Ca2+ mobilization and phosphorylation studies of membrane currents induced by AlF4- in Xenopus oocytes

Cellular Signalling
C MoonM B Djamgoz

Abstract

We have examined the electrophysiological responses induced by aluminium fluoride (AlF4-) and carbachol in Xenopus oocytes. Application of AlF4- induced Ca(2+)-dependent oscillatory and smooth Cl- currents. Pre-treatment of oocytes with microinjected guanosine 5'-O-(2-thiodiphosphate) diminished the currents, indicating that the effect of AlF4- occurred through G-protein activation. Confocal imaging of intracellular Ca2+ clearly demonstrated that AlF4- could increase the internal Ca2+ concentration in oocytes in the absence of external Ca2+. A protein kinase (PK) activator (4-beta-phorbol 12,13-dibutyrate) decreased the AlF4(-)-induced membrane currents, whereas a PK inhibitor (staurosporine) caused an increase. On the other hand, the protein phosphatase inhibitor (okadaic acid) showed little effect. Although the effects of the phosphorylating/dephosphorylating agents on the carbachol-induced currents were qualitatively similar to the case of AlF4-, some quantitative differences was noted. The results are discussed in terms of the signaling pathways involving muscarinic receptors and G-protein(s) in Xenopus oocytes.

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Citations

Apr 13, 2001·European Journal of Pharmacology·M C BrenoZ P Picarelli
Sep 20, 2000·Journal of Receptor and Signal Transduction Research·B BufeW Meyerhof
Mar 31, 2010·The Journal of Physiology·Juan Martinez-PinnaAndrés Morales
Jul 25, 2003·American Journal of Physiology. Cell Physiology·Seon Ah ChongJeong Taeg Seo
Sep 30, 1999·Microscopy Research and Technique·S A Stricker, M Whitaker
Oct 16, 1999·Biochimica Et Biophysica Acta·W Weber

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