G Protein-Coupled Estrogen Receptor Regulates Actin Cytoskeleton Dynamics to Impair Cell Polarization

Frontiers in Cell and Developmental Biology
Dariusz LachowskiArmando E Del Río Hernández

Abstract

Mechanical forces regulate cell functions through multiple pathways. G protein-coupled estrogen receptor (GPER) is a seven-transmembrane receptor that is ubiquitously expressed across tissues and mediates the acute cellular response to estrogens. Here, we demonstrate an unidentified role of GPER as a cellular mechanoregulator. G protein-coupled estrogen receptor signaling controls the assembly of stress fibers, the dynamics of the associated focal adhesions, and cell polarization via RhoA GTPase (RhoA). G protein-coupled estrogen receptor activation inhibits F-actin polymerization and subsequently triggers a negative feedback that transcriptionally suppresses the expression of monomeric G-actin. Given the broad expression of GPER and the range of cytoskeletal changes modulated by this receptor, our findings position GPER as a key player in mechanotransduction.

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Citations

Jan 29, 2021·Frontiers in Cell and Developmental Biology·Islam M SaadeldinRamya A Sindi

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Methods Mentioned

BETA
GTPases
GTPase
nuclear translocation
fluorescence recovery after photobleaching
fluorescence recovery after the photobleaching
scanning electron microscopy
AFM
Transfection
immunoprecipitation assay
protein assay

Software Mentioned

AFM
Fiji
elements AR
Image Studio Lite
GraphPad
NIS
Prism

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