G-protein-coupled receptors in Saccharomyces cerevisiae: high-throughput screening assays for drug discovery

Trends in Biotechnology
M H Pausch

Abstract

G-protein-coupled receptors are an important class of therapeutic drug targets by virtue of their roles in the regulation of diverse cellular functions. Recent advances in the expression of heterologous G-protein-coupled receptors in the yeast Saccharomyces cerevisiae have led to the development of sensitive and selective assays of their ligand-induced activation. Implementation of this new technology in the high-throughput screening of compound libraries has enabled the discovery of novel ligands for the G-protein-coupled somatostatin receptor. This article describes the broad applicability of the technology and its use in drug discovery.

References

Feb 14, 1992·Biochemical and Biophysical Research Communications·H J HuangT T Kuo
Jan 1, 1991·Annual Review of Biochemistry·H G DohlmanR J Lefkowitz
Jan 1, 1995·Life Sciences·Y C PatelC B Srikant
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·K J BlumerC A Lange-Carter
Jan 25, 1996·Nature·D G LambrightP B Sigler
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·J B KonopkaP Dube
Jul 1, 1996·Trends in Pharmacological Sciences·G MilliganS Rees
Nov 7, 1996·Nature·J R Broach, J Thorner
Nov 1, 1996·Trends in Biotechnology·C G Tate, R Grisshammer
Jan 1, 1997·Journal of Receptor and Signal Transduction Research·E M KajkowskiB A Ozenberger
Feb 14, 1997·The Journal of Biological Chemistry·H G Dohlman, J Thorner

❮ Previous
Next ❯

Citations

Apr 12, 2008·Purinergic Signalling·Torsten SchönebergAngela Schulz
Apr 22, 1999·Trends in Pharmacological Sciences·G Milligan, S Rees
Nov 20, 1998·Current Opinion in Biotechnology·H Reiländer, H M Weiss
Feb 19, 2000·Current Opinion in Biotechnology·K G Fleming
Jan 28, 2003·Drug Discovery Today·Chandra L Tucker
Nov 18, 1998·Current Opinion in Chemical Biology·P B Fernandes
Jul 14, 1998·Immunotechnology : an International Journal of Immunological Engineering·H R HoogenboomR C Roovers
Oct 4, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiqin MaoHanfa Zou
Mar 1, 2012·Acta Pharmacologica Sinica·Xiao-long TangMing-yao Liu
Aug 1, 2008·Nature Methods·Bruce R ConklinBryan L Roth
Nov 26, 2010·The Journal of Biological Chemistry·Ines LiebscherAngela Schulz
Jul 21, 2001·EMBO Reports·M VerseleJ M Thevelein
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Alan WiseStephen Rees
May 7, 2010·The AAPS Journal·Akihiko OzawaWesley K Kroeze
Sep 1, 2004·Pharmacogenomics·Inge MertensLiliane Schoofs
Jul 30, 2014·Applied Microbiology and Biotechnology·Anita EmmerstorferHarald Pichler
Sep 28, 2010·Annual Review of Pharmacology and Toxicology·John A Allen, Bryan L Roth
May 9, 2007·Nature Chemical Biology·Venkat RadhikaDanny N Dhanasekaran
Nov 25, 2003·Journal of Receptor and Signal Transduction Research·Edith Pajot-AugyIan Connerton
May 21, 2015·ACS Synthetic Biology·Kuntal MukherjeePamela Peralta-Yahya
Mar 7, 2013·Molecular Microbiology·Kentaro Furukawa, Stefan Hohmann
Feb 22, 2005·Biochemical and Biophysical Research Communications·Dezhong YinHsien-Yu Wang
Aug 30, 2005·Trends in Pharmacological Sciences·Margot W Beukers, Adriaan P Ijzerman
Apr 30, 2005·Trends in Pharmacological Sciences·Willem H Mager, Joris Winderickx
Nov 27, 2010·The International Journal of Biochemistry & Cell Biology·Mario Andrea Marchisio, Fabian Rudolf
Feb 18, 2014·International Journal for Parasitology. Drugs and Drug Resistance·Martha J LarsenTeresa M Kubiak
Dec 3, 2014·Analytical Biochemistry·Yosuke FukutaniMasafumi Yohda
Dec 2, 2009·The Journal of Cell Biology·Karmella A Haynes, Pamela A Silver
Jan 16, 2002·Journal of Receptor and Signal Transduction Research·M Dani
Jun 21, 2006·Journal of Psychopharmacology·Wesley K Kroeze, Bryan L Roth
Oct 11, 2003·FEBS Letters·Douglas A GriffithDieter Oesterhelt
Aug 10, 2006·Biotechnology Journal·Christine SchwimmerJean-Paul di Rago

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.