PMID: 7544531Aug 1, 1995Paper

G protein G alpha i-2 inhibits outwardly rectifying chloride channels in human airway epithelial cells

The American Journal of Physiology
E M SchwiebertB A Stanton

Abstract

Previously we demonstrated that the heterotrimeric G protein, G alpha i-2, inhibits cystic fibrosis transmembrane conductance regulator (CFTR) chloride (Cl-) channels in human airway epithelial cells (E. M. Schwiebert, F. Gesek, L. Ercolani, C. Wjasow, D. C. Gruenert, and B. A. Stanton. Am. J. Physiol. 267 (Cell Physiol. 36): C272-C281, 1994, and E. M. Schwiebert, N. L. Kizer, D. C. Gruenert, and B. A. Stanton. Proc. Natl. Acad. Sci. USA 89: 10623-10627, 1992). The goal of the present study was to determine if G proteins also regulate outwardly rectifying Cl- channels (ORCC), a distinct class of Cl- channels regulated defectively by protein kinase A (PKA) in cystic fibrosis (CF). To this end, we used the patch-clamp technique to study ORCC in a normal human airway epithelial cell line (9HTEo-) that expresses CFTR and ORCC. Stimulation of G proteins with GTP and GTP gamma S decreased the single-channel open probability (Po) of ORCC, whereas inhibition of G proteins by GDP beta S increased the Po. Moreover, pertussis toxin (PTX), an uncoupler of Gi and G(o) subclasses of heterotrimeric G proteins, also increased the Po. Purified G alpha i-2 decreased the Po. In contrast, other PTX-sensitive G proteins, G alpha i-1, G alpha i-3, a...Continue Reading

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Citations

Nov 13, 2008·Pflügers Archiv : European journal of physiology·Diana FariaKarl Kunzelmann
Aug 1, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·O MignenB J Harvey
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
Feb 15, 2001·American Journal of Physiology. Cell Physiology·J R BroughmanB D Schultz
Aug 4, 1998·The American Journal of Physiology·B JovovD J Benos
Jun 25, 1999·The Journal of Physiology·T LuE F Shibata
Mar 10, 2001·Biochemical and Biophysical Research Communications·R SchreiberK Kunzelmann
Apr 17, 2007·Blood Cells, Molecules & Diseases·Gaëtan DecherfSerge L Y Thomas
Mar 17, 2010·Blood Cells, Molecules & Diseases·Edyta GlogowskaSerge L Y Thomas

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