G-protein mRNA levels during adipocyte differentiation.

Biochemical and Biophysical Research Communications
D C WatkinsC C Malbon

Abstract

G-protein-mediated transmembrane signaling in 3T3-L1 cells is modulated by differentiation. The regulation of G-protein expression in differentiating 3T3-L1 cells was probed at the level of mRNA by DNA-excess solution hybridization. Pertussis toxin-catalyzed ADP-ribosylation of G-protein alpha-subunits increased as fibroblasts differentiate to adipocytes. Steady-state levels of mRNA for Gi alpha 2 and Go alpha, in contrast, declined sharply. Immunoblotting with antipeptide antibodies specific for Gi alpha 2, too, revealed a decline in the steady-state expression of this pertussis toxin substrate. ADP-ribosylation of Gs alpha by cholera toxin was less in the adipocyte than fibroblast. Analysis by immunoblotting revealed only a modest decline in Gs alpha. Analysis of mRNA levels also demonstrated a decline for Gs alpha. mRNA levels for the G beta-subunits rose initially (25%) on day 1, declined from day 1 to day 3, and remained 25% lower in adipocytes than in fibroblasts. In 3T3-L1 adipocytes the molar amounts of subunit mRNAs were: 60.6 (Gs alpha); 2.1 (Gi alpha 2); and 1.5 (Go alpha) amol/microgram total cellular RNA. In rat fat cells these mRNA levels were 19.4 (Gs alpha); 7.0 (Gi alpha 2); and 2.3 (Go alpha). These data demon...Continue Reading

References

May 15, 1989·The Biochemical Journal·M RosC C Malbon
Jan 1, 1988·Trends in Pharmacological Sciences·C C MalbonD C Watkins
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
May 12, 1988·Nature·E J Neer, D E Clapham

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Citations

Aug 1, 1993·Journal of Cellular Biochemistry·N McFarlane-AndersonN Bégin-Heick
Aug 28, 1995·Brain Research. Developmental Brain Research·S AndreopoulosJ J Warsh
Aug 1, 1993·The Journal of Clinical Investigation·S I SenkforT Berl
Feb 13, 1999·The Journal of Biological Chemistry·A M Aragay, M W Quick
Jun 13, 1998·The Journal of Biological Chemistry·X LiuH Y Wang
Nov 5, 1999·The Journal of Biological Chemistry·H y Wang, C C Malbon
Aug 1, 1991·Biochemical Medicine and Metabolic Biology·O ShinoharaC Kubota

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